Basic Information
ID: ALA3888582
Type: Binding
Description: LCMS Assay: The MGAT enzyme reactions were performed in Corning FALCON® 96-well Polypropylene plates, in a total volume of 60 μL of 50 mM Potassium Phosphate buffer pH 7.4, containing a final concentration of 100 μM 2-oleoylglycerol, 15 μM oleoyl-Coenzyme A and 0.0013 μg/μL Human or Mouse MGAT-2 or 0.0026 μg/μL Rat recombinant MGAT-2 membranes expressed in Sf9 cells. Assay plates were run through a fully automated robotics system and shaken for 5 seconds every minute for a total 10 minutes. The reactions were then quenched with 120 μL of ice cold methanol containing 1 μg/mL 1,2-distearoyl-rac-glycerol as the internal standard. Plates were shaken for 2 minutes and spun down to remove protein precipitation. After the spin, samples were transferred to LC/MS compatible PCR plates. For LC/MS analysis, a ThermoFisher Surveyor pump, utilizing a Waters Symmetry C8, 50×2.1 mm column, was used for the chromatography of enzyme products. The buffer system consists of 0.1% formic acid in water with a mobile phase consisting 0.1% formic acid in methanol. The shallow gradient is 90-100% mobile phase in 0.2 min with a total run time of 2.3 min. The first 0.5 minutes of each injection was diverted to waste to eliminate the presence of Phosphate buffer in the enzymatic reaction. The column was run at 0.6 mL/min and a temperature of 65° C.
Cell type: Sf9
Activity Charts
Bioactivity
Activity Types for Assay ALA3888582
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 684.60 | 6.13 | 6.13 |
| 672.59 | 5.99 | 5.99 |
| 661.67 | 5.59 | 5.59 |
| 650.62 | 5.05 | 5.05 |
| 666.66 | 5.68 | 5.68 |
| 652.68 | 6.15 | 6.15 |
| 640.60 | 5.95 | 5.95 |
| 626.55 | 4.28 | 4.28 |
| 649.61 | 4.93 | 4.93 |
| 640.58 | 4.66 | 4.66 |
| 648.67 | 6.29 | 6.29 |
| 622.59 | 4.21 | 4.21 |
| 635.63 | 5.15 | 5.15 |
| 640.58 | 4.67 | 4.67 |
| 620.57 | 4.28 | 4.28 |
| 620.57 | 4.76 | 4.76 |
| 674.70 | 6.83 | 6.83 |
| 638.56 | 4.42 | 4.42 |
| 620.62 | 5.08 | 5.08 |
| 578.53 | 4.52 | 4.52 |
| 607.57 | 4.28 | 4.28 |
| 612.52 | 3.89 | 3.89 |
| 634.60 | 4.67 | 4.67 |
| 648.63 | 4.74 | 4.74 |
| 592.56 | 4.91 | 4.91 |
| 662.65 | 5.13 | 5.13 |
| 634.60 | 4.35 | 4.35 |
| 672.69 | 6.48 | 6.48 |
| 620.61 | 5.69 | 5.69 |
| 614.54 | 4.13 | 4.13 |
| 648.63 | 5.06 | 5.06 |
| 632.63 | 4.91 | 4.91 |
| 649.65 | 5.91 | 5.91 |
| 579.52 | 3.77 | 3.77 |
| 666.62 | 5.20 | 5.20 |
| 623.57 | 4.07 | 4.07 |
| 626.55 | 4.75 | 4.75 |
| 622.56 | 4.84 | 4.84 |
| 583.70 | 5.30 | 5.30 |
| 658.67 | 5.39 | 5.39 |
| 622.59 | 4.53 | 4.53 |
| 678.70 | 5.77 | 5.77 |
| 640.57 | 5.14 | 5.14 |
| 610.60 | 5.15 | 5.15 |
| 652.59 | 4.81 | 4.81 |
| 618.60 | 4.83 | 4.83 |
| 558.58 | 4.38 | 4.38 |
| 606.59 | 4.65 | 4.65 |
| 638.66 | 5.28 | 5.28 |
| 608.56 | 3.82 | 3.82 |
| 566.64 | 4.97 | 4.97 |
| 666.62 | 4.88 | 4.88 |
| 655.61 | 5.22 | 5.22 |
| 664.67 | 5.48 | 5.48 |
| 668.63 | 5.13 | 5.13 |
| 650.66 | 6.08 | 6.08 |
| 484.54 | 3.76 | 3.76 |
| 666.61 | 5.68 | 5.68 |
| 658.56 | 5.60 | 5.60 |
| 650.61 | 6.01 | 6.01 |
| 598.59 | 4.84 | 4.84 |
| 628.59 | 5.29 | 5.29 |
| 652.59 | 5.29 | 5.29 |
| 632.50 | 5.29 | 5.29 |
| 607.57 | 4.37 | 4.37 |
| 687.61 | 5.44 | 5.44 |
| 606.59 | 5.30 | 5.30 |
| 564.63 | 4.77 | 4.77 |
| 624.58 | 4.66 | 4.66 |
| 580.63 | 3.81 | 3.81 |
| 594.53 | 4.22 | 4.22 |
| 636.64 | 5.69 | 5.69 |
| 632.50 | 5.41 | 5.41 |
| 637.62 | 5.82 | 5.82 |
| 660.68 | 5.61 | 5.61 |
| 620.61 | 5.69 | 5.69 |
| 650.61 | 5.98 | 5.98 |
| 637.67 | 5.85 | 5.85 |
| 604.57 | 5.06 | 5.06 |
| 639.01 | 5.71 | 5.71 |
| 646.65 | 5.22 | 5.22 |
| 620.61 | 5.51 | 5.51 |
| 630.51 | 4.82 | 4.82 |
| 648.62 | 5.54 | 5.54 |
| 632.62 | 5.84 | 5.84 |
| 652.59 | 4.49 | 4.49 |
| 660.56 | 6.07 | 6.07 |
| 626.55 | 4.10 | 4.10 |
| 392.52 | 2.54 | 2.54 |
| 583.70 | 5.30 | 5.30 |
| 564.55 | 4.69 | 4.69 |
| 484.54 | 3.62 | 3.62 |
| 536.52 | 3.91 | 3.91 |