Basic Information
ID: ALA3888593
Type: Binding
Description: Caliper Mobility-Shift Assay: To a 96-well plate, 20 μL, of 10 μM FL-cGMP as the substrate was added; then 1 μL, of solution of the compound in DMSO or DMSO solution without compound was added, and then 29 μL of 1.38 ng/μL PDE-5A enzyme buffer (100 mM Hepes pH 7.5, 5 mM MgCl2, 0.002% Brij-35) was added. The maximum final concentration of the compound was 10 μM. The plate was incubated at 30° C. for 1 h, and 20 μL of 70 μM EDTA was then added to stop the reaction. 26 μL of solution of each well was transferred to a 384-well plate.
Activity Charts
Bioactivity
Activity Types for Assay ALA3888593
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 483.96 | 2.43 | 2.43 |
| 494.98 | 2.89 | 2.89 |
| 487.00 | 2.27 | 2.27 |
| 478.00 | 4.67 | 4.67 |
| 494.00 | 3.85 | 3.85 |
| 471.99 | 3.24 | 3.24 |
| 465.95 | 2.93 | 2.93 |
| 484.99 | 2.61 | 2.61 |
| 466.93 | 2.32 | 2.32 |
| 486.02 | 3.77 | 3.77 |
| 495.97 | 2.29 | 2.29 |
| 478.98 | 4.07 | 4.07 |
| 481.96 | 4.28 | 4.28 |
| 517.03 | 1.63 | 1.63 |
| 464.96 | 3.53 | 3.53 |
| 470.02 | 4.66 | 4.66 |
| 514.07 | 2.72 | 2.72 |
| 500.04 | 4.16 | 4.16 |
| 492.02 | 5.06 | 5.06 |
| 463.97 | 4.14 | 4.14 |
| 479.97 | 3.24 | 3.24 |
| 501.03 | 2.80 | 2.80 |
| 479.97 | 3.46 | 3.46 |
| 494.00 | 3.50 | 3.50 |
| 502.02 | 2.60 | 2.60 |