Basic Information
ID: ALA3888594
Type: Binding
Description: Enzyme Assay: The test compound or solvent was incubated at 25° C. for 15 min with enzyme solution (35 μg/mL) in Tris-HCl buffer of pH 7.5. 1 μM cGMP and 0.01 μM [3H] cGMP were then added to activate the reaction. The mixture was incubated for 20 min. The reaction was stopped at 100° C. By addition of snake venom nucleotidase, the product [3H] GMP was converted into [3H] Guanosine, which was separated with AG1-X2 resin. Then the amount of [3H] Guanosine was measured.
Activity Charts
Bioactivity
Activity Types for Assay ALA3888594
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 455.53 | 2.73 | 2.73 |
| 471.99 | 3.24 | 3.24 |
| 465.95 | 2.93 | 2.93 |
| 486.02 | 3.77 | 3.77 |
| 451.57 | 2.90 | 2.90 |
| 514.07 | 2.72 | 2.72 |
| 463.97 | 4.14 | 4.14 |