Basic Information
ID: ALA3888596
Type: Binding
Description: Caliper Mobility-Shift Assay: To a 96-well plate, 20 μL of 10 μM FL-cGMP as the substrate was added; then 1 μL solution of the compound in DMSO or DMSO solution without compound was added; and then 29 μL of 0.28 ng/μL PDE-11 enzyme buffer (100 mM Hepes pH 7.5, 5 mM MgCl2, 0.002% Brij-35) was added. The maximum final concentration of the compound was 10 μM. The plate was incubated at 30° C. for 1 h, and 20 μL of 70 μM EDTA was then added to stop the reaction. 26 μL of solution of each well was transferred to a 384-well plate.
Activity Charts
Bioactivity
Activity Types for Assay ALA3888596
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 487.00 | 2.27 | 2.27 |
| 471.99 | 3.24 | 3.24 |
| 465.95 | 2.93 | 2.93 |
| 484.99 | 2.61 | 2.61 |
| 523.04 | 3.00 | 3.00 |
| 500.04 | 4.02 | 4.02 |
| 481.96 | 4.28 | 4.28 |
| 508.03 | 4.24 | 4.24 |
| 464.96 | 3.53 | 3.53 |
| 470.02 | 4.66 | 4.66 |
| 500.05 | 2.38 | 2.38 |
| 514.07 | 2.72 | 2.72 |
| 485.03 | 3.74 | 3.74 |
| 479.97 | 3.24 | 3.24 |
| 471.01 | 2.94 | 2.94 |