[35S]TBPS Binding Assay: Briefly, aliquots of membrane solution (0.5 mg/mL final protein concentration in assay) were incubated with 5 μM GABA, 2 nM [...

ID: ALA3888767

Type: Binding

Description: [35S]TBPS Binding Assay: Briefly, aliquots of membrane solution (0.5 mg/mL final protein concentration in assay) were incubated with 5 μM GABA, 2 nM [35S]TBPS (45−120 Ci/mmol), and 5 μL aliquots of steroid in DMSO solution (final assay concentrations ranged from 1 nM to 10 μM), and brought to a final volume of 1 mL with 200 mM NaCl, 50 mM potassium phosphate buffer, pH 7.4. Control binding was defined as binding observed in the presence of 0.5% DMSO and the absence of steroid. Nonspecific binding was defined as binding observed in the presence of 200 μM picrotoxinin and ranged from 6.1 to 14.3% of total binding. Assay tubes were incubated for 2 h at room temperature.

Format: BAO_0000019

Target:  GABA-A receptor; alpha-1/beta-2/gamma-2(ALA2095172)

Document:  ALA3886830

BindingDB Source:  8248