Basic Information
ID: ALA3888893
Type: Binding
Description: Luciferase Assay: The Huh-7 cells transfected with HCV replicons system were seeded into 96-well plates (8,000 cells in 125 μL/well) respectively; each test compound was diluted to desired concentration using 5-fold serial dilutions protocol, 10 doses in duplicate, and added to wells with POD™ 810 Plate Assembler. The plates were incubated in a CO2 incubator for 72 hours; after that, 40 μL of Luciferase assay substrate (Promega Bright-Glo) was added to each well, and detected by a chemiluminescence detection system (Topcount Microplate Scintillation and Luminescence Counter) 5 minutes later; the EC50 (half-maximal effective concentration, concentration for 50% of maximal effect) values of test compounds were analyzed by GraphPad Prism software, respectively. In this paper, experiments were repeated twice and set the holes without compounds as negative control.
Cell type: Huh-7
Activity Charts
Bioactivity
Activity Types for Assay ALA3888893
EC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 925.19 | 9.35 | 9.35 |
| 855.01 | 7.12 | 7.12 |
| 910.13 | 9.00 | 9.00 |
| 917.15 | 8.46 | 8.46 |
| 935.14 | 8.39 | 8.39 |
| 969.15 | 7.75 | 7.75 |
| 964.14 | 8.53 | 8.53 |
| 924.16 | 8.92 | 8.92 |
| 930.14 | 9.24 | 9.24 |
| 913.09 | 7.24 | 7.24 |
| 963.19 | 9.17 | 9.17 |
| 999.08 | 8.12 | 8.12 |
| 1091.37 | ||
| 939.17 | 8.34 | 8.34 |
| 961.12 | 8.68 | 8.68 |
| 991.25 | 10.24 | 10.24 |
| 911.12 | 8.39 | 8.39 |
| 888.08 | 7.27 | 7.27 |
| 979.15 | 9.83 | 9.83 |
| 925.19 | 9.35 | 9.35 |
| 948.16 | 7.20 | 7.20 |
| 963.19 | 8.88 | 8.88 |
| 973.19 | 9.55 | 9.55 |
| 934.15 | 9.58 | 9.58 |
| 952.17 | 7.54 | 7.54 |
| 947.17 | 7.81 | 7.81 |
| 967.22 | 9.67 | 9.67 |
| 930.09 | 7.93 | 7.93 |
| 939.17 | 9.17 | 9.17 |
| 859.04 | 6.46 | 6.46 |
| 911.12 | 8.39 | 8.39 |
| 938.18 | 9.96 | 9.96 |
| 940.18 | 8.24 | 8.24 |
| 963.19 | 9.17 | 9.17 |