Basic Information
ID: ALA3888896
Type: Binding
Description: Kinase Assay: Sphingosine kinase activity was assessed as described previously (Kharel, Y., Lee, S., Snyder, A. H., Sheasley-O'Neill, S. L., Morris, M. A., Setiady, Y., Zhu, R., Zigler, M. A., Burcin, T. L., Ley, K., Tung, K. S. K., Engelhard, V. H., Macdonald, T. L. and Lynch, K. R. Sphingosine kinase 2 is required for modulation of lymphocyte traffic by FTY720. J Biological Chemistry 280: 36865-36872 (2005)). High levels of human sphingosine kinase type 1 (mSK1) and mouse sphingosine kinase type 2 (mSK2) were expressed in Sf9 insect cells by infection with cognate baculoviruses. Crude homogenates were incubated with γ-[32P]ATP and 10 micromolar D-erythro-sphingosine in the presence of 100 micromolar concentrations of test compounds for 20 minutes at 37 C. The product, radiolabeled S1P, was isolated by extraction into organic solvents after acidification and displaced by thin layer chromatography.
Activity Charts
Bioactivity
Activity Types for Assay ALA3888896
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 367.58 | 5.11 | 5.11 |
| 401.60 | 5.50 | 5.50 |
| 443.63 | 5.63 | 5.63 |
| 423.56 | 4.86 | 4.86 |
| 373.54 | 4.72 | 4.72 |
| 429.61 | 4.79 | 4.79 |
| 339.52 | 4.33 | 4.33 |
| 415.58 | 4.40 | 4.40 |
| 379.59 | 5.42 | 5.42 |
| 333.43 | 3.04 | 3.04 |
| 395.63 | 5.89 | 5.89 |
| 429.61 | 5.24 | 5.24 |
| 415.62 | 5.94 | 5.94 |
| 373.54 | 5.23 | 5.23 |
| 443.63 | 5.13 | 5.13 |
| 359.51 | 4.38 | 4.38 |
| 421.59 | 5.67 | 5.67 |
| 415.62 | 6.40 | 6.40 |
| 361.49 | 3.82 | 3.82 |
| 443.68 | 6.72 | 6.72 |
| 395.59 | 4.35 | 4.35 |
| 407.56 | 5.28 | 5.28 |
| 417.59 | 5.39 | 5.39 |
| 331.46 | 3.60 | 3.60 |
| 359.51 | 4.83 | 4.83 |
| 387.57 | 5.16 | 5.16 |
| 415.58 | 4.85 | 4.85 |
| 443.68 | 7.18 | 7.18 |
| 387.57 | 5.11 | 5.11 |
| 387.57 | 5.62 | 5.62 |
| 389.54 | 4.61 | 4.61 |
| 381.56 | 3.96 | 3.96 |
| 345.49 | 3.94 | 3.94 |
| 481.68 | 5.88 | 5.88 |
| 353.55 | 4.86 | 4.86 |
| 401.60 | 5.50 | 5.50 |