Basic Information
ID: ALA3888910
Type: Binding
Description: Cellular Uptake Assay : URAT1 (SLC22A12) activity was evaluated in a cellular uptake assay using a 96-well plate with stably transfected URAT-1/CHO cells. 3H-orotate was used as the test transport agent, which was measured in a liquid scintillation counter, using benzbromarone as a positive control, and DMSO and non-transfected CHO cells as negative controls (Solvo Biotechnology, Boston, Mass.). Over 7 concentrations (range, 0.01 to 150 μM), a semi-log plot (percent relative transport of oratate vs. time) was generated to determine the concentration at which 50% inhibition was observed (i.e., the IC50).
Cell type: CHO
Activity Charts
Bioactivity
Activity Types for Assay ALA3888910
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 263.28 | -0.23 | -0.23 |
| 247.21 | 0.02 | 0.02 |
| 263.21 | -0.27 | -0.27 |
| 279.28 | 1.10 | 1.10 |