ID: ALA3888966
Type: Binding
Description: In Vitro Assay: CHO-K1 CXCR7 b-arrestin cells are detached from culture dishes with a cell dissociation buffer (Invitrogen, #13151-014) and collected in growing medium (F12 HAMS 90% (v/v)/FCS 10% (v/v), Penicilin/streptomycin 1% (v/v)). 5000 cells per well (in 20 μl) are seeded in a 384 well plate (white-walled, clear bottom; BD Falcon #353274). The plate is incubated at 37° C./5% CO2 for 24 hours. Medium is then replaced by 20 μl OPTIMEM (Invitrogen #31985) for 3 to 4 hours. Test compounds are dissolved at 10 mM in DMSO and serially diluted in DMSO to 200× of the final concentration for dose response curves. Compounds are then diluted 1:33.3 in HBSS1×. 5 μl/well of HBSS1×/20 mM HEPES/0.2% BSA are added to the assay plate followed by addition of 5 μl/well of diluted compounds. CXCL12 (Peprotech #300-28A) may be used as a reference agonist. The plate is incubated for 90 minutes at 37° C. 12 μl of detection reagent (Path Hunter Detection Kit, DiscoveRx, #93-0001) is transferred to the assay plate and to the plate is incubated for 1 hour at room temperature. Luminescent signal is read in a microplate reader (FLUOstar Optima, bmg).
Bioactivity
Activity Types for Assay ALA3888966
EC50
Parent Molecular Weight | ALogP | Polar Surface Area |
---|---|---|
428.96 | 4.11 | 4.11 |
510.00 | 5.67 | 5.67 |
462.52 | 4.48 | 4.48 |
441.55 | 4.21 | 4.21 |
394.52 | 3.38 | 3.38 |
495.73 | 6.17 | 6.17 |
476.54 | 4.68 | 4.68 |
458.97 | 4.40 | 4.40 |
476.54 | 4.79 | 4.79 |
480.51 | 4.62 | 4.62 |
439.64 | 5.26 | 5.26 |
463.62 | 4.92 | 4.92 |
455.00 | 4.65 | 4.65 |
507.44 | 5.44 | 5.44 |
502.58 | 5.57 | 5.57 |
448.49 | 4.09 | 4.09 |
505.56 | 5.76 | 5.76 |
532.61 | 4.62 | 4.62 |
502.49 | 5.79 | 5.79 |
496.44 | 5.77 | 5.77 |
399.56 | 4.08 | 4.08 |
343.47 | 2.94 | 2.94 |
462.52 | 4.34 | 4.34 |
433.60 | 4.48 | 4.48 |
455.55 | 4.88 | 4.88 |
434.58 | 4.26 | 4.26 |
463.62 | 4.54 | 4.54 |
478.52 | 3.72 | 3.72 |
502.58 | 5.57 | 5.57 |
486.66 | 5.02 | 5.02 |
447.62 | 5.22 | 5.22 |
494.44 | 5.44 | 5.44 |
473.62 | 5.04 | 5.04 |
488.46 | 5.91 | 5.91 |
492.54 | 4.49 | 4.49 |
492.54 | 4.49 | 4.49 |
435.57 | 3.70 | 3.70 |
476.54 | 4.87 | 4.87 |
434.58 | 4.56 | 4.56 |
461.53 | 5.08 | 5.08 |
439.58 | 4.77 | 4.77 |
462.98 | 4.52 | 4.52 |
509.72 | 5.22 | 5.22 |
427.98 | 4.72 | 4.72 |
461.65 | 5.72 | 5.72 |
444.58 | 4.47 | 4.47 |
415.58 | 3.57 | 3.57 |
476.54 | 4.87 | 4.87 |
392.50 | 3.13 | 3.13 |
422.57 | 4.08 | 4.08 |
458.99 | 3.74 | 3.74 |
438.57 | 3.67 | 3.67 |
472.00 | 5.39 | 5.39 |
424.55 | 3.90 | 3.90 |
463.62 | 5.17 | 5.17 |
439.99 | 4.86 | 4.86 |
438.55 | 4.13 | 4.13 |
392.50 | 3.13 | 3.13 |
492.54 | 3.70 | 3.70 |
487.65 | 4.80 | 4.80 |
395.51 | 2.86 | 2.86 |
488.55 | 5.01 | 5.01 |
474.53 | 4.62 | 4.62 |
436.56 | 3.23 | 3.23 |
434.58 | 3.96 | 3.96 |
427.98 | 4.67 | 4.67 |
409.53 | 2.78 | 2.78 |
470.62 | 5.15 | 5.15 |
380.49 | 2.94 | 2.94 |
489.99 | 5.50 | 5.50 |
425.60 | 4.66 | 4.66 |
464.49 | 3.27 | 3.27 |
420.56 | 3.87 | 3.87 |
435.57 | 3.95 | 3.95 |
400.57 | 2.87 | 2.87 |
444.58 | 4.47 | 4.47 |
433.60 | 5.16 | 5.16 |
508.97 | 5.28 | 5.28 |
435.61 | 4.73 | 4.73 |
505.56 | 5.76 | 5.76 |
461.53 | 5.08 | 5.08 |
395.48 | 3.57 | 3.57 |
383.54 | 3.70 | 3.70 |
474.53 | 4.62 | 4.62 |
447.62 | 4.56 | 4.56 |
496.44 | 5.77 | 5.77 |
434.58 | 4.34 | 4.34 |
463.62 | 4.54 | 4.54 |
448.03 | 4.71 | 4.71 |
409.53 | 3.32 | 3.32 |
449.60 | 4.61 | 4.61 |
462.64 | 5.17 | 5.17 |
419.57 | 4.09 | 4.09 |
448.61 | 4.86 | 4.86 |
492.54 | 4.49 | 4.49 |
470.56 | 4.55 | 4.55 |
470.01 | 4.22 | 4.22 |
435.57 | 3.95 | 3.95 |
395.51 | 2.86 | 2.86 |
455.99 | 4.04 | 4.04 |
412.92 | 3.48 | 3.48 |
425.55 | 4.40 | 4.40 |
499.45 | 4.76 | 4.76 |
470.01 | 4.23 | 4.23 |
441.55 | 4.21 | 4.21 |
434.58 | 4.11 | 4.11 |
439.58 | 4.77 | 4.77 |
502.06 | 6.00 | 6.00 |
438.55 | 4.13 | 4.13 |
447.62 | 5.22 | 5.22 |
472.00 | 4.93 | 4.93 |
448.61 | 4.61 | 4.61 |
385.90 | 3.55 | 3.55 |
436.56 | 3.61 | 3.61 |
444.58 | 4.61 | 4.61 |
473.42 | 4.22 | 4.22 |
494.44 | 5.44 | 5.44 |
445.97 | 4.40 | 4.40 |
438.57 | 3.78 | 3.78 |
455.55 | 4.88 | 4.88 |
489.45 | 5.30 | 5.30 |
425.55 | 4.51 | 4.51 |
484.04 | 5.26 | 5.26 |
474.07 | 5.62 | 5.62 |
462.52 | 4.48 | 4.48 |
434.58 | 4.56 | 4.56 |
448.03 | 4.71 | 4.71 |
436.94 | 3.98 | 3.98 |
410.52 | 3.59 | 3.59 |
449.60 | 4.26 | 4.26 |
533.98 | 6.04 | 6.04 |
470.06 | 5.38 | 5.38 |
462.64 | 4.67 | 4.67 |
387.52 | 2.79 | 2.79 |
420.56 | 3.99 | 3.99 |
461.99 | 5.12 | 5.12 |
466.03 | 5.11 | 5.11 |
455.00 | 4.65 | 4.65 |
442.00 | 4.65 | 4.65 |
459.59 | 4.48 | 4.48 |
468.04 | 5.81 | 5.81 |
462.98 | 4.52 | 4.52 |
452.56 | 3.25 | 3.25 |
448.61 | 4.86 | 4.86 |
489.45 | 5.30 | 5.30 |
490.57 | 5.26 | 5.26 |
413.61 | 4.43 | 4.43 |
411.52 | 4.20 | 4.20 |
417.51 | 3.03 | 3.03 |
454.01 | 5.25 | 5.25 |
544.45 | 6.32 | 6.32 |
451.60 | 4.07 | 4.07 |
478.64 | 3.76 | 3.76 |
506.54 | 5.15 | 5.15 |
433.60 | 4.91 | 4.91 |
447.62 | 5.22 | 5.22 |
412.51 | 3.60 | 3.60 |
451.59 | 5.05 | 5.05 |
467.59 | 4.75 | 4.75 |
437.56 | 4.74 | 4.74 |
411.59 | 4.48 | 4.48 |
423.56 | 3.33 | 3.33 |
434.58 | 4.30 | 4.30 |
503.69 | 5.16 | 5.16 |
396.47 | 2.96 | 2.96 |
474.53 | 4.62 | 4.62 |
476.45 | 5.30 | 5.30 |
490.53 | 3.80 | 3.80 |
482.07 | 5.89 | 5.89 |
447.62 | 5.22 | 5.22 |
411.93 | 4.08 | 4.08 |
447.62 | 5.16 | 5.16 |
454.01 | 5.11 | 5.11 |
502.58 | 5.40 | 5.40 |
434.58 | 4.30 | 4.30 |
424.55 | 3.47 | 3.47 |
452.57 | 4.40 | 4.40 |
438.55 | 4.13 | 4.13 |
530.72 | 5.79 | 5.79 |
392.50 | 3.13 | 3.13 |
465.61 | 5.11 | 5.11 |
451.59 | 5.05 | 5.05 |
488.46 | 5.91 | 5.91 |
492.02 | 5.13 | 5.13 |
426.54 | 3.86 | 3.86 |
422.57 | 4.16 | 4.16 |
501.59 | 6.18 | 6.18 |
479.02 | 5.27 | 5.27 |
455.00 | 4.65 | 4.65 |
408.55 | 3.93 | 3.93 |
492.54 | 4.49 | 4.49 |
470.62 | 5.15 | 5.15 |
409.53 | 3.32 | 3.32 |
474.53 | 4.62 | 4.62 |
433.60 | 4.53 | 4.53 |
467.59 | 4.75 | 4.75 |
421.55 | 3.39 | 3.39 |
468.04 | 5.18 | 5.18 |
424.55 | 2.96 | 2.96 |
455.00 | 4.65 | 4.65 |
420.56 | 3.87 | 3.87 |
452.57 | 4.44 | 4.44 |
476.54 | 4.87 | 4.87 |
481.60 | 3.41 | 3.41 |
481.71 | 5.96 | 5.96 |
488.46 | 5.91 | 5.91 |
469.03 | 5.21 | 5.21 |
468.04 | 5.56 | 5.56 |
421.58 | 4.56 | 4.56 |
555.56 | 6.64 | 6.64 |
478.52 | 3.72 | 3.72 |
419.57 | 4.60 | 4.60 |
420.56 | 3.99 | 3.99 |
476.54 | 4.87 | 4.87 |
451.59 | 5.05 | 5.05 |
448.61 | 4.86 | 4.86 |
496.44 | 5.77 | 5.77 |
518.71 | 3.75 | 3.75 |
490.57 | 5.04 | 5.04 |
474.53 | 4.62 | 4.62 |
489.66 | 4.60 | 4.60 |
502.70 | 5.96 | 5.96 |
447.62 | 5.22 | 5.22 |
450.58 | 4.00 | 4.00 |
510.12 | 6.53 | 6.53 |
448.61 | 4.69 | 4.69 |
455.00 | 4.65 | 4.65 |
493.70 | 5.09 | 5.09 |
434.58 | 4.26 | 4.26 |
496.05 | 5.19 | 5.19 |
434.58 | 4.56 | 4.56 |
447.62 | 5.22 | 5.22 |
488.46 | 5.91 | 5.91 |
502.58 | 5.40 | 5.40 |
469.63 | 5.75 | 5.75 |
434.58 | 4.30 | 4.30 |
417.55 | 4.27 | 4.27 |
454.55 | 3.37 | 3.37 |
471.62 | 3.47 | 3.47 |
425.55 | 4.51 | 4.51 |
449.60 | 4.61 | 4.61 |
450.58 | 4.00 | 4.00 |
479.62 | 4.62 | 4.62 |
503.57 | 5.50 | 5.50 |
435.57 | 3.70 | 3.70 |
447.00 | 4.32 | 4.32 |
433.60 | 4.48 | 4.48 |
448.61 | 4.86 | 4.86 |
394.52 | 3.38 | 3.38 |
458.61 | 5.03 | 5.03 |
414.58 | 3.83 | 3.83 |
469.63 | 5.75 | 5.75 |
436.60 | 4.33 | 4.33 |
434.58 | 4.30 | 4.30 |
425.55 | 4.51 | 4.51 |
469.01 | 4.58 | 4.58 |
515.62 | 6.11 | 6.11 |
488.46 | 5.91 | 5.91 |
454.01 | 5.25 | 5.25 |
464.49 | 3.27 | 3.27 |
435.57 | 3.95 | 3.95 |
488.03 | 5.09 | 5.09 |
433.58 | 4.53 | 4.53 |
413.95 | 4.33 | 4.33 |
434.58 | 4.25 | 4.25 |
444.96 | 3.35 | 3.35 |
408.55 | 3.77 | 3.77 |
474.53 | 4.62 | 4.62 |
483.66 | 5.68 | 5.68 |
409.53 | 3.04 | 3.04 |
457.98 | 5.00 | 5.00 |
488.55 | 5.01 | 5.01 |
502.58 | 5.40 | 5.40 |
470.62 | 5.15 | 5.15 |
442.63 | 4.07 | 4.07 |
439.63 | 4.97 | 4.97 |
454.01 | 5.25 | 5.25 |
442.51 | 3.72 | 3.72 |
433.60 | 4.91 | 4.91 |
490.57 | 5.26 | 5.26 |
425.62 | 4.87 | 4.87 |
506.54 | 5.11 | 5.11 |
488.55 | 5.01 | 5.01 |
476.54 | 4.87 | 4.87 |
449.60 | 4.26 | 4.26 |
445.57 | 4.01 | 4.01 |
490.05 | 5.86 | 5.86 |
502.58 | 5.26 | 5.26 |
439.63 | 5.00 | 5.00 |
434.58 | 4.30 | 4.30 |
493.65 | 4.93 | 4.93 |
445.57 | 4.01 | 4.01 |
476.45 | 5.30 | 5.30 |
409.53 | 3.32 | 3.32 |
476.54 | 4.79 | 4.79 |
479.62 | 4.62 | 4.62 |
450.61 | 4.68 | 4.68 |
442.54 | 3.23 | 3.23 |
421.55 | 3.39 | 3.39 |
425.55 | 4.51 | 4.51 |
478.59 | 3.78 | 3.78 |
449.60 | 4.26 | 4.26 |
407.56 | 3.99 | 3.99 |
476.45 | 5.30 | 5.30 |
487.57 | 5.62 | 5.62 |
473.66 | 5.09 | 5.09 |
438.55 | 4.13 | 4.13 |
419.57 | 4.47 | 4.47 |
468.04 | 5.50 | 5.50 |
448.61 | 4.69 | 4.69 |
448.61 | 4.69 | 4.69 |
492.54 | 4.11 | 4.11 |
385.55 | 3.80 | 3.80 |
420.56 | 3.91 | 3.91 |
462.52 | 4.48 | 4.48 |
455.00 | 4.65 | 4.65 |
463.62 | 4.54 | 4.54 |
446.95 | 4.25 | 4.25 |
414.58 | 3.83 | 3.83 |
423.53 | 4.35 | 4.35 |
469.03 | 4.96 | 4.96 |
459.59 | 5.35 | 5.35 |
440.98 | 4.26 | 4.26 |
505.71 | 5.40 | 5.40 |
449.60 | 4.61 | 4.61 |
438.57 | 3.78 | 3.78 |
357.48 | 2.96 | 2.96 |
436.60 | 4.55 | 4.55 |
451.59 | 5.05 | 5.05 |
476.54 | 4.73 | 4.73 |
494.08 | 5.74 | 5.74 |
385.55 | 3.94 | 3.94 |
408.55 | 3.77 | 3.77 |
505.66 | 3.83 | 3.83 |
429.95 | 3.57 | 3.57 |
451.59 | 5.05 | 5.05 |
357.50 | 3.33 | 3.33 |
537.57 | 6.25 | 6.25 |
439.64 | 5.26 | 5.26 |
444.58 | 4.61 | 4.61 |
424.52 | 3.74 | 3.74 |
541.41 | 5.24 | 5.24 |
408.55 | 3.72 | 3.72 |
462.52 | 4.48 | 4.48 |
436.56 | 3.09 | 3.09 |
417.55 | 4.27 | 4.27 |
491.04 | 5.41 | 5.41 |
438.55 | 4.13 | 4.13 |
426.54 | 3.80 | 3.80 |
422.57 | 3.97 | 3.97 |
476.54 | 4.79 | 4.79 |
438.57 | 3.40 | 3.40 |
436.60 | 4.33 | 4.33 |
453.89 | 4.48 | 4.48 |
472.00 | 5.39 | 5.39 |
469.03 | 5.21 | 5.21 |
434.58 | 4.30 | 4.30 |
397.56 | 4.09 | 4.09 |
460.04 | 5.31 | 5.31 |
478.52 | 3.72 | 3.72 |
478.52 | 3.45 | 3.45 |
459.99 | 4.79 | 4.79 |
438.57 | 3.78 | 3.78 |
439.99 | 4.86 | 4.86 |
492.02 | 5.13 | 5.13 |
506.57 | 4.09 | 4.09 |
455.99 | 4.10 | 4.10 |
462.64 | 5.25 | 5.25 |
462.64 | 4.67 | 4.67 |
435.57 | 3.93 | 3.93 |
450.58 | 4.00 | 4.00 |
413.61 | 4.72 | 4.72 |
421.55 | 3.39 | 3.39 |
462.52 | 4.48 | 4.48 |
406.53 | 3.48 | 3.48 |
475.68 | 5.73 | 5.73 |
477.98 | 4.93 | 4.93 |
487.57 | 5.62 | 5.62 |
442.63 | 4.07 | 4.07 |
460.62 | 4.87 | 4.87 |
434.58 | 4.30 | 4.30 |
455.00 | 4.57 | 4.57 |
491.04 | 5.41 | 5.41 |
422.57 | 4.16 | 4.16 |
478.52 | 3.45 | 3.45 |
451.59 | 5.05 | 5.05 |
422.57 | 4.08 | 4.08 |
476.54 | 4.73 | 4.73 |
422.57 | 4.16 | 4.16 |
434.58 | 3.92 | 3.92 |
440.52 | 2.98 | 2.98 |
459.99 | 4.79 | 4.79 |
485.03 | 4.66 | 4.66 |
437.56 | 4.74 | 4.74 |
475.68 | 5.90 | 5.90 |
488.55 | 5.01 | 5.01 |
508.97 | 5.28 | 5.28 |
476.54 | 4.68 | 4.68 |
468.04 | 5.14 | 5.14 |
392.50 | 3.13 | 3.13 |
458.61 | 5.03 | 5.03 |
420.44 | 3.31 | 3.31 |
491.68 | 4.84 | 4.84 |
371.51 | 3.30 | 3.30 |
461.53 | 5.08 | 5.08 |
436.60 | 4.33 | 4.33 |
456.59 | 4.76 | 4.76 |
424.52 | 3.74 | 3.74 |
427.57 | 3.37 | 3.37 |
455.55 | 4.88 | 4.88 |
459.59 | 4.48 | 4.48 |
492.02 | 5.13 | 5.13 |
409.53 | 2.78 | 2.78 |
476.54 | 4.68 | 4.68 |
422.57 | 4.16 | 4.16 |
456.54 | 4.11 | 4.11 |
504.60 | 5.60 | 5.60 |
472.00 | 5.39 | 5.39 |
488.55 | 5.01 | 5.01 |
394.52 | 3.38 | 3.38 |
459.63 | 5.09 | 5.09 |
396.47 | 2.96 | 2.96 |
459.59 | 4.48 | 4.48 |
439.58 | 4.77 | 4.77 |
443.98 | 3.82 | 3.82 |
447.62 | 4.92 | 4.92 |
422.57 | 4.08 | 4.08 |
462.98 | 4.52 | 4.52 |
448.61 | 4.86 | 4.86 |
455.00 | 4.65 | 4.65 |
508.97 | 5.28 | 5.28 |
422.57 | 4.02 | 4.02 |
424.52 | 3.42 | 3.42 |
451.59 | 5.30 | 5.30 |
460.98 | 4.64 | 4.64 |
456.59 | 4.76 | 4.76 |
409.53 | 3.16 | 3.16 |
462.52 | 4.48 | 4.48 |
420.56 | 3.91 | 3.91 |
441.55 | 4.21 | 4.21 |
455.00 | 4.65 | 4.65 |
424.52 | 3.42 | 3.42 |
456.03 | 5.21 | 5.21 |
474.53 | 4.48 | 4.48 |
455.55 | 4.88 | 4.88 |
480.51 | 4.57 | 4.57 |
438.55 | 4.13 | 4.13 |
425.60 | 4.66 | 4.66 |
468.04 | 5.18 | 5.18 |
458.00 | 4.14 | 4.14 |
420.56 | 3.99 | 3.99 |
476.54 | 4.79 | 4.79 |
406.53 | 3.38 | 3.38 |
472.00 | 5.39 | 5.39 |
464.64 | 4.98 | 4.98 |
476.54 | 4.87 | 4.87 |
468.04 | 5.56 | 5.56 |
490.57 | 5.04 | 5.04 |
488.46 | 5.91 | 5.91 |
471.62 | 3.47 | 3.47 |
406.53 | 3.52 | 3.52 |
488.03 | 5.61 | 5.61 |
455.00 | 4.65 | 4.65 |
442.99 | 4.50 | 4.50 |
434.46 | 3.70 | 3.70 |
475.42 | 4.91 | 4.91 |
492.54 | 4.11 | 4.11 |
544.45 | 6.32 | 6.32 |
472.63 | 5.09 | 5.09 |
448.49 | 3.95 | 3.95 |
441.55 | 4.21 | 4.21 |
426.54 | 3.91 | 3.91 |
474.07 | 5.62 | 5.62 |
458.61 | 5.08 | 5.08 |
442.99 | 4.31 | 4.31 |
433.60 | 4.91 | 4.91 |
490.57 | 5.04 | 5.04 |
478.52 | 3.72 | 3.72 |
448.61 | 4.56 | 4.56 |
428.51 | 2.84 | 2.84 |
516.61 | 5.96 | 5.96 |
434.58 | 4.30 | 4.30 |
420.56 | 3.77 | 3.77 |
469.03 | 5.21 | 5.21 |
424.55 | 3.01 | 3.01 |
490.57 | 5.12 | 5.12 |
505.56 | 5.76 | 5.76 |
459.59 | 4.48 | 4.48 |
468.04 | 5.18 | 5.18 |
422.57 | 4.16 | 4.16 |
406.53 | 3.52 | 3.52 |
526.08 | 6.83 | 6.83 |
437.56 | 4.74 | 4.74 |
475.68 | 5.80 | 5.80 |
422.57 | 4.16 | 4.16 |
422.57 | 4.08 | 4.08 |
425.96 | 4.47 | 4.47 |
437.63 | 4.72 | 4.72 |