Assay Report Card

Basic Information

ID: ALA3888966

Type: Binding

Description: In Vitro Assay: CHO-K1 CXCR7 b-arrestin cells are detached from culture dishes with a cell dissociation buffer (Invitrogen, #13151-014) and collected in growing medium (F12 HAMS 90% (v/v)/FCS 10% (v/v), Penicilin/streptomycin 1% (v/v)). 5000 cells per well (in 20 μl) are seeded in a 384 well plate (white-walled, clear bottom; BD Falcon #353274). The plate is incubated at 37° C./5% CO2 for 24 hours. Medium is then replaced by 20 μl OPTIMEM (Invitrogen #31985) for 3 to 4 hours. Test compounds are dissolved at 10 mM in DMSO and serially diluted in DMSO to 200× of the final concentration for dose response curves. Compounds are then diluted 1:33.3 in HBSS1×. 5 μl/well of HBSS1×/20 mM HEPES/0.2% BSA are added to the assay plate followed by addition of 5 μl/well of diluted compounds. CXCL12 (Peprotech #300-28A) may be used as a reference agonist. The plate is incubated for 90 minutes at 37° C. 12 μl of detection reagent (Path Hunter Detection Kit, DiscoveRx, #93-0001) is transferred to the assay plate and to the plate is incubated for 1 hour at room temperature. Luminescent signal is read in a microplate reader (FLUOstar Optima, bmg).

Activity Charts

Compound Summary

Parent Molecular WeightALogPPolar Surface Area
428.964.114.11
510.005.675.67
462.524.484.48
441.554.214.21
394.523.383.38
495.736.176.17
476.544.684.68
458.974.404.40
476.544.794.79
480.514.624.62
439.645.265.26
463.624.924.92
455.004.654.65
507.445.445.44
502.585.575.57
448.494.094.09
505.565.765.76
532.614.624.62
502.495.795.79
496.445.775.77
399.564.084.08
343.472.942.94
462.524.344.34
433.604.484.48
455.554.884.88
434.584.264.26
463.624.544.54
478.523.723.72
502.585.575.57
486.665.025.02
447.625.225.22
494.445.445.44
473.625.045.04
488.465.915.91
492.544.494.49
492.544.494.49
435.573.703.70
476.544.874.87
434.584.564.56
461.535.085.08
439.584.774.77
462.984.524.52
509.725.225.22
427.984.724.72
461.655.725.72
444.584.474.47
415.583.573.57
476.544.874.87
392.503.133.13
422.574.084.08
458.993.743.74
438.573.673.67
472.005.395.39
424.553.903.90
463.625.175.17
439.994.864.86
438.554.134.13
392.503.133.13
492.543.703.70
487.654.804.80
395.512.862.86
488.555.015.01
474.534.624.62
436.563.233.23
434.583.963.96
427.984.674.67
409.532.782.78
470.625.155.15
380.492.942.94
489.995.505.50
425.604.664.66
464.493.273.27
420.563.873.87
435.573.953.95
400.572.872.87
444.584.474.47
433.605.165.16
508.975.285.28
435.614.734.73
505.565.765.76
461.535.085.08
395.483.573.57
383.543.703.70
474.534.624.62
447.624.564.56
496.445.775.77
434.584.344.34
463.624.544.54
448.034.714.71
409.533.323.32
449.604.614.61
462.645.175.17
419.574.094.09
448.614.864.86
492.544.494.49
470.564.554.55
470.014.224.22
435.573.953.95
395.512.862.86
455.994.044.04
412.923.483.48
425.554.404.40
499.454.764.76
470.014.234.23
441.554.214.21
434.584.114.11
439.584.774.77
502.066.006.00
438.554.134.13
447.625.225.22
472.004.934.93
448.614.614.61
385.903.553.55
436.563.613.61
444.584.614.61
473.424.224.22
494.445.445.44
445.974.404.40
438.573.783.78
455.554.884.88
489.455.305.30
425.554.514.51
484.045.265.26
474.075.625.62
462.524.484.48
434.584.564.56
448.034.714.71
436.943.983.98
410.523.593.59
449.604.264.26
533.986.046.04
470.065.385.38
462.644.674.67
387.522.792.79
420.563.993.99
461.995.125.12
466.035.115.11
455.004.654.65
442.004.654.65
459.594.484.48
468.045.815.81
462.984.524.52
452.563.253.25
448.614.864.86
489.455.305.30
490.575.265.26
413.614.434.43
411.524.204.20
417.513.033.03
454.015.255.25
544.456.326.32
451.604.074.07
478.643.763.76
506.545.155.15
433.604.914.91
447.625.225.22
412.513.603.60
451.595.055.05
467.594.754.75
437.564.744.74
411.594.484.48
423.563.333.33
434.584.304.30
503.695.165.16
396.472.962.96
474.534.624.62
476.455.305.30
490.533.803.80
482.075.895.89
447.625.225.22
411.934.084.08
447.625.165.16
454.015.115.11
502.585.405.40
434.584.304.30
424.553.473.47
452.574.404.40
438.554.134.13
530.725.795.79
392.503.133.13
465.615.115.11
451.595.055.05
488.465.915.91
492.025.135.13
426.543.863.86
422.574.164.16
501.596.186.18
479.025.275.27
455.004.654.65
408.553.933.93
492.544.494.49
470.625.155.15
409.533.323.32
474.534.624.62
433.604.534.53
467.594.754.75
421.553.393.39
468.045.185.18
424.552.962.96
455.004.654.65
420.563.873.87
452.574.444.44
476.544.874.87
481.603.413.41
481.715.965.96
488.465.915.91
469.035.215.21
468.045.565.56
421.584.564.56
555.566.646.64
478.523.723.72
419.574.604.60
420.563.993.99
476.544.874.87
451.595.055.05
448.614.864.86
496.445.775.77
518.713.753.75
490.575.045.04
474.534.624.62
489.664.604.60
502.705.965.96
447.625.225.22
450.584.004.00
510.126.536.53
448.614.694.69
455.004.654.65
493.705.095.09
434.584.264.26
496.055.195.19
434.584.564.56
447.625.225.22
488.465.915.91
502.585.405.40
469.635.755.75
434.584.304.30
417.554.274.27
454.553.373.37
471.623.473.47
425.554.514.51
449.604.614.61
450.584.004.00
479.624.624.62
503.575.505.50
435.573.703.70
447.004.324.32
433.604.484.48
448.614.864.86
394.523.383.38
458.615.035.03
414.583.833.83
469.635.755.75
436.604.334.33
434.584.304.30
425.554.514.51
469.014.584.58
515.626.116.11
488.465.915.91
454.015.255.25
464.493.273.27
435.573.953.95
488.035.095.09
433.584.534.53
413.954.334.33
434.584.254.25
444.963.353.35
408.553.773.77
474.534.624.62
483.665.685.68
409.533.043.04
457.985.005.00
488.555.015.01
502.585.405.40
470.625.155.15
442.634.074.07
439.634.974.97
454.015.255.25
442.513.723.72
433.604.914.91
490.575.265.26
425.624.874.87
506.545.115.11
488.555.015.01
476.544.874.87
449.604.264.26
445.574.014.01
490.055.865.86
502.585.265.26
439.635.005.00
434.584.304.30
493.654.934.93
445.574.014.01
476.455.305.30
409.533.323.32
476.544.794.79
479.624.624.62
450.614.684.68
442.543.233.23
421.553.393.39
425.554.514.51
478.593.783.78
449.604.264.26
407.563.993.99
476.455.305.30
487.575.625.62
473.665.095.09
438.554.134.13
419.574.474.47
468.045.505.50
448.614.694.69
448.614.694.69
492.544.114.11
385.553.803.80
420.563.913.91
462.524.484.48
455.004.654.65
463.624.544.54
446.954.254.25
414.583.833.83
423.534.354.35
469.034.964.96
459.595.355.35
440.984.264.26
505.715.405.40
449.604.614.61
438.573.783.78
357.482.962.96
436.604.554.55
451.595.055.05
476.544.734.73
494.085.745.74
385.553.943.94
408.553.773.77
505.663.833.83
429.953.573.57
451.595.055.05
357.503.333.33
537.576.256.25
439.645.265.26
444.584.614.61
424.523.743.74
541.415.245.24
408.553.723.72
462.524.484.48
436.563.093.09
417.554.274.27
491.045.415.41
438.554.134.13
426.543.803.80
422.573.973.97
476.544.794.79
438.573.403.40
436.604.334.33
453.894.484.48
472.005.395.39
469.035.215.21
434.584.304.30
397.564.094.09
460.045.315.31
478.523.723.72
478.523.453.45
459.994.794.79
438.573.783.78
439.994.864.86
492.025.135.13
506.574.094.09
455.994.104.10
462.645.255.25
462.644.674.67
435.573.933.93
450.584.004.00
413.614.724.72
421.553.393.39
462.524.484.48
406.533.483.48
475.685.735.73
477.984.934.93
487.575.625.62
442.634.074.07
460.624.874.87
434.584.304.30
455.004.574.57
491.045.415.41
422.574.164.16
478.523.453.45
451.595.055.05
422.574.084.08
476.544.734.73
422.574.164.16
434.583.923.92
440.522.982.98
459.994.794.79
485.034.664.66
437.564.744.74
475.685.905.90
488.555.015.01
508.975.285.28
476.544.684.68
468.045.145.14
392.503.133.13
458.615.035.03
420.443.313.31
491.684.844.84
371.513.303.30
461.535.085.08
436.604.334.33
456.594.764.76
424.523.743.74
427.573.373.37
455.554.884.88
459.594.484.48
492.025.135.13
409.532.782.78
476.544.684.68
422.574.164.16
456.544.114.11
504.605.605.60
472.005.395.39
488.555.015.01
394.523.383.38
459.635.095.09
396.472.962.96
459.594.484.48
439.584.774.77
443.983.823.82
447.624.924.92
422.574.084.08
462.984.524.52
448.614.864.86
455.004.654.65
508.975.285.28
422.574.024.02
424.523.423.42
451.595.305.30
460.984.644.64
456.594.764.76
409.533.163.16
462.524.484.48
420.563.913.91
441.554.214.21
455.004.654.65
424.523.423.42
456.035.215.21
474.534.484.48
455.554.884.88
480.514.574.57
438.554.134.13
425.604.664.66
468.045.185.18
458.004.144.14
420.563.993.99
476.544.794.79
406.533.383.38
472.005.395.39
464.644.984.98
476.544.874.87
468.045.565.56
490.575.045.04
488.465.915.91
471.623.473.47
406.533.523.52
488.035.615.61
455.004.654.65
442.994.504.50
434.463.703.70
475.424.914.91
492.544.114.11
544.456.326.32
472.635.095.09
448.493.953.95
441.554.214.21
426.543.913.91
474.075.625.62
458.615.085.08
442.994.314.31
433.604.914.91
490.575.045.04
478.523.723.72
448.614.564.56
428.512.842.84
516.615.965.96
434.584.304.30
420.563.773.77
469.035.215.21
424.553.013.01
490.575.125.12
505.565.765.76
459.594.484.48
468.045.185.18
422.574.164.16
406.533.523.52
526.086.836.83
437.564.744.74
475.685.805.80
422.574.164.16
422.574.084.08
425.964.474.47
437.634.724.72