Basic Information
ID: ALA3888976
Type: Binding
Description: Cell Proliferation Inhibition Assay: In order to make human PIK3 CA mutation-positive breast cancer cell line MDA-MB-453 cells become 500 cells per well in a 384 well non-adhesive plate (Lipidure-Coat plate AT-384; manufactured by NOF Corporation), 36 μl/well was seeded in Leibovitz's L-15 medium including 10% fetal bovine serum (manufactured by Life technologies Corp.), followed by culturing at 37° C. overnight in the presence of CO2. The following day, the test compound (the test compound had 11 steps in a final concentration from 10000 nM to 0.1 nM), and DMSO which is a solvent for the test compound as a negative control were diluted with the medium, and 4 μl was added to cells. Thereafter, the cells were cultured for 4 days at 37° C. in the absence of CO2, and a cytometric reagent (CellTiter-Glo Luminescent Cell Viability assay manufactured by Promega Corporation) was added. The resultant product was stirred for 30 minutes, and then measurement was carried out using a luminescence measurement apparatus
Organism: Homo sapiens
Cell type: Cancer cell lines
Activity Charts
Bioactivity
Activity Types for Assay ALA3888976
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 591.68 | 5.93 | 5.93 |
| 577.73 | 4.58 | 4.58 |
| 606.69 | 5.33 | 5.33 |
| 592.67 | 5.09 | 5.09 |
| 604.66 | 4.63 | 4.63 |
| 592.67 | 5.09 | 5.09 |
| 593.65 | 4.49 | 4.49 |
| 606.69 | 5.33 | 5.33 |
| 593.65 | 4.72 | 4.72 |
| 577.73 | 4.58 | 4.58 |
| 606.69 | 5.33 | 5.33 |
| 549.68 | 4.18 | 4.18 |
| 592.67 | 5.32 | 5.32 |
| 607.68 | 5.04 | 5.04 |
| 592.67 | 5.25 | 5.25 |
| 592.67 | 5.09 | 5.09 |
| 563.71 | 4.19 | 4.19 |
| 610.66 | 5.46 | 5.46 |