Basic Information
ID: ALA3888978
Type: Binding
Description: In Vitro Biological Assay: An in vitro biological assay that can be used includes the steps of (1) mixing a histone substrate (e.g., an isolated histone sample for a histone or modified histone of interest, or an isolated oligonucleosome substrate) with recombinant DOT1L enzyme (e.g., recombinant protein containing amino acids 1-416); (2) adding a candidate compound of the invention to this mixture; (3) adding non-radioactive and 3H-labeled S-Adenosyl methionine (SAM) to start the reaction; (4) adding excessive amount of non-radioactive SAM to stop the reaction; (4) washing off the free non-incorporated 3H-SAM; and (5) detecting the quantity of 3H-labeled histone substrate by any methods known in the art (e.g., by a PerkinElmer TopCount platereader).
Format: BAO_0000357
Target: Histone-lysine N-methyltransferase, H3 lysine-79 specific(ALA1795117)
Document: ALA3886905
BindingDB Source: 8384
Activity Charts
Bioactivity
Activity Types for Assay ALA3888978
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 562.72 | 3.32 | 3.32 |
| 562.72 | 3.32 | 3.32 |
| 561.73 | 3.93 | 3.93 |
| 590.61 | 2.92 | 2.92 |
| 589.62 | 3.53 | 3.53 |
| 590.61 | 2.92 | 2.92 |
| 609.05 | 3.70 | 3.70 |
| 533.68 | 3.15 | 3.15 |
| 531.71 | 3.81 | 3.81 |