Basic Information
ID: ALA3889128
Type: Binding
Description: In Vitro Assay: Method 1: Recombinant human factor D (expressed in E. coli and purified using standard methods) at 10 nM concentration is incubated with test compound at various concentrations for 1 hour at room temperature in 0.1 M Hepes buffer, pH 7.5, containing 1 mM MgCl2, 1 M NaCl and 0.05% CHAPS. A synthetic substrate Z-Lys-thiobenzyl and 2,4-dinitrobenzenesulfonyl-fluoresceine are added to final concentrations of 200 μM and 25 μM, respectively. The increase in fluorescence is recorded at excitation of 485 nm and emission at 535 nm in a microplate spectrofluorimeter. IC50 values are calculated from percentage of inhibition of complement factor D-activity as a function of test compound concentration.
Activity Charts
Bioactivity
Activity Types for Assay ALA3889128
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 427.48 | 1.89 | 1.89 |
| 449.46 | 1.08 | 1.08 |
| 450.45 | 0.47 | 0.47 |
| 447.90 | 2.16 | 2.16 |
| 443.48 | 0.87 | 0.87 |
| 453.40 | 1.66 | 1.66 |
| 412.47 | 2.61 | 2.61 |
| 413.45 | 2.00 | 2.00 |
| 442.50 | 1.52 | 1.52 |
| 427.48 | 1.89 | 1.89 |
| 427.48 | 1.81 | 1.81 |
| 420.42 | 0.85 | 0.85 |
| 413.45 | 1.50 | 1.50 |
| 414.44 | 0.90 | 0.90 |
| 428.47 | 1.21 | 1.21 |
| 428.47 | 1.21 | 1.21 |
| 413.45 | 2.94 | 2.94 |
| 439.49 | 2.04 | 2.04 |
| 419.43 | 1.45 | 1.45 |
| 442.50 | 1.46 | 1.46 |
| 427.48 | 1.89 | 1.89 |
| 520.57 | 2.98 | 2.98 |