Basic Information
ID: ALA3889157
Type: Binding
Description: ELISA Assay: CVF-Bb complex prepared from purified cobra venom factor (1 μM), recombinant human complement factor B (expressed in drosophila cells and purified using standard methods) and human complement factor D (expressed in E. Coli, refolded and purified using standard methods). CVF-Bb complex at 3 nM concentration was incubated with test compound at various concentrations for 1 hour at room temperature in PBS pH 7.4 containing 10 mM MgCl2 and 0.05% (w/v) CHAPS. Human complement C3 substrate purified from plasma was added to a final concentration of 1 μM. After 1 hour incubation at room temperature, the enzyme reaction was stopped by addition of a cocktail of concentrated pan-protease inhibitors. The product of the reaction, C3a, was quantified by means of an enzyme-linked-immunosorbent assay.
Format: BAO_0000219
Tissue: Plasma
Target: Complement factor D(ALA2176771)
Document: ALA3886970
BindingDB Source: 8471
Activity Charts
Bioactivity
Activity Types for Assay ALA3889157
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 476.51 | 2.99 | 2.99 |
| 448.54 | 2.17 | 2.17 |
| 486.45 | 3.09 | 3.09 |
| 408.46 | 1.72 | 1.72 |
| 482.49 | 3.34 | 3.34 |
| 417.90 | 2.50 | 2.50 |
| 517.57 | 3.85 | 3.85 |
| 510.55 | 4.12 | 4.12 |
| 468.46 | 2.95 | 2.95 |
| 489.51 | 3.07 | 3.07 |
| 466.47 | 2.52 | 2.52 |
| 401.44 | 1.98 | 1.98 |
| 434.52 | 1.78 | 1.78 |
| 503.54 | 3.46 | 3.46 |
| 486.52 | 2.63 | 2.63 |
| 416.46 | 0.76 | 0.76 |
| 482.56 | 2.37 | 2.37 |
| 537.60 | 2.38 | 2.38 |
| 401.44 | 1.98 | 1.98 |
| 507.57 | 2.76 | 2.76 |
| 462.35 | 2.61 | 2.61 |
| 486.52 | 2.63 | 2.63 |
| 543.00 | 3.87 | 3.87 |
| 490.54 | 3.38 | 3.38 |
| 475.48 | 2.68 | 2.68 |
| 550.56 | 3.90 | 3.90 |
| 536.53 | 3.51 | 3.51 |
| 493.54 | 2.37 | 2.37 |
| 506.54 | 2.62 | 2.62 |
| 429.50 | 2.76 | 2.76 |
| 450.47 | 2.81 | 2.81 |
| 468.53 | 2.50 | 2.50 |
| 459.52 | 2.39 | 2.39 |
| 564.58 | 4.29 | 4.29 |
| 483.55 | 1.76 | 1.76 |
| 500.55 | 3.02 | 3.02 |
| 417.90 | 2.50 | 2.50 |
| 462.49 | 2.60 | 2.60 |
| 504.57 | 3.77 | 3.77 |
| 521.60 | 3.15 | 3.15 |
| 471.92 | 3.38 | 3.38 |
| 480.50 | 2.82 | 2.82 |
| 454.51 | 2.11 | 2.11 |
| 468.53 | 2.37 | 2.37 |
| 515.41 | 2.73 | 2.73 |
| 488.95 | 2.76 | 2.76 |
| 521.60 | 3.02 | 3.02 |
| 530.58 | 2.52 | 2.52 |
| 514.58 | 3.29 | 3.29 |
| 544.60 | 2.91 | 2.91 |
| 533.40 | 2.87 | 2.87 |
| 538.58 | 4.34 | 4.34 |
| 500.55 | 3.02 | 3.02 |
| 444.47 | 1.70 | 1.70 |
| 522.50 | 3.13 | 3.13 |
| 474.56 | 2.48 | 2.48 |
| 472.50 | 2.25 | 2.25 |
| 493.54 | 2.24 | 2.24 |
| 472.50 | 2.25 | 2.25 |
| 472.50 | 2.25 | 2.25 |
| 518.54 | 2.85 | 2.85 |
| 554.52 | 3.44 | 3.44 |
| 474.58 | 2.58 | 2.58 |
| 468.53 | 2.42 | 2.42 |
| 490.49 | 2.39 | 2.39 |
| 548.59 | 3.99 | 3.99 |
| 550.56 | 3.18 | 3.18 |
| 490.49 | 2.39 | 2.39 |
| 445.50 | 2.00 | 2.00 |
| 461.53 | 1.84 | 1.84 |
| 537.06 | 3.86 | 3.86 |
| 514.63 | 3.21 | 3.21 |
| 500.55 | 2.85 | 2.85 |
| 468.53 | 2.42 | 2.42 |
| 444.47 | 1.70 | 1.70 |
| 479.52 | 1.98 | 1.98 |
| 506.94 | 2.90 | 2.90 |
| 539.59 | 3.16 | 3.16 |
| 488.95 | 2.76 | 2.76 |
| 478.53 | 2.09 | 2.09 |
| 549.65 | 3.79 | 3.79 |
| 482.56 | 2.88 | 2.88 |
| 446.53 | 1.92 | 1.92 |
| 504.51 | 3.04 | 3.04 |
| 515.57 | 2.68 | 2.68 |
| 522.50 | 3.13 | 3.13 |
| 523.57 | 2.00 | 2.00 |
| 460.54 | 2.17 | 2.17 |
| 479.52 | 1.98 | 1.98 |
| 544.56 | 4.69 | 4.69 |
| 482.56 | 2.71 | 2.71 |
| 507.57 | 2.63 | 2.63 |
| 500.55 | 2.90 | 2.90 |
| 512.56 | 2.99 | 2.99 |
| 551.58 | 4.42 | 4.42 |
| 486.52 | 2.51 | 2.51 |
| 460.54 | 2.17 | 2.17 |
| 488.95 | 2.76 | 2.76 |
| 540.62 | 3.77 | 3.77 |
| 479.52 | 1.98 | 1.98 |