Assay Report Card

Basic Information

ID: ALA3889157

Type: Binding

Description: ELISA Assay: CVF-Bb complex prepared from purified cobra venom factor (1 μM), recombinant human complement factor B (expressed in drosophila cells and purified using standard methods) and human complement factor D (expressed in E. Coli, refolded and purified using standard methods). CVF-Bb complex at 3 nM concentration was incubated with test compound at various concentrations for 1 hour at room temperature in PBS pH 7.4 containing 10 mM MgCl2 and 0.05% (w/v) CHAPS. Human complement C3 substrate purified from plasma was added to a final concentration of 1 μM. After 1 hour incubation at room temperature, the enzyme reaction was stopped by addition of a cocktail of concentrated pan-protease inhibitors. The product of the reaction, C3a, was quantified by means of an enzyme-linked-immunosorbent assay.

Tissue: Plasma

Activity Charts

Bioactivity

Activity Types for Assay ALA3889157

IC50

Total(131)

Compound Summary

Parent Molecular Weight	ALogP	Polar Surface Area
476.51	2.99	2.99
508.60	3.06	3.06
448.54	2.17	2.17
468.53	2.15	2.15
439.52	1.28	1.28
486.45	3.09	3.09
408.46	1.72	1.72
482.49	3.34	3.34
417.90	2.50	2.50
517.57	3.85	3.85
480.54	2.29	2.29
510.55	4.12	4.12
476.55	1.57	1.57
468.46	2.95	2.95
508.60	3.15	3.15
489.51	3.07	3.07
466.47	2.52	2.52
401.44	1.98	1.98
434.52	1.78	1.78
503.54	3.46	3.46
486.52	2.63	2.63
416.46	0.76	0.76
482.56	2.37	2.37
498.53	2.43	2.43
462.53	1.18	1.18
432.50	1.41	1.41
494.57	2.90	2.90
537.60	2.38	2.38
401.44	1.98	1.98
494.57	2.90	2.90
507.57	2.76	2.76
462.35	2.61	2.61
486.52	2.63	2.63
466.52	2.25	2.25
460.55	2.33	2.33
543.00	3.87	3.87
490.54	3.38	3.38
475.48	2.68	2.68
550.56	3.90	3.90
446.53	1.94	1.94
536.53	3.51	3.51
493.54	2.37	2.37
506.54	2.62	2.62
429.50	2.76	2.76
494.57	2.67	2.67
460.55	2.33	2.33
450.47	2.81	2.81
468.53	2.50	2.50
459.52	2.39	2.39
564.58	4.29	4.29
432.50	1.55	1.55
483.55	1.76	1.76
404.45	0.77	0.77
508.60	3.29	3.29
418.47	1.16	1.16
474.58	2.53	2.53
500.55	3.02	3.02
417.90	2.50	2.50
462.49	2.60	2.60
432.50	1.55	1.55
504.57	3.77	3.77
480.54	2.51	2.51
521.60	3.15	3.15
471.92	3.38	3.38
480.50	2.82	2.82
454.51	2.11	2.11
468.53	2.37	2.37
515.41	2.73	2.73
488.95	2.76	2.76
563.63	2.65	2.65
521.60	3.02	3.02
530.58	2.52	2.52
514.58	3.29	3.29
544.60	2.91	2.91
533.40	2.87	2.87
538.58	4.34	4.34
500.55	3.02	3.02
444.47	1.70	1.70
522.50	3.13	3.13
474.56	2.48	2.48
472.50	2.25	2.25
526.59	3.38	3.38
493.54	2.24	2.24
472.50	2.25	2.25
472.50	2.25	2.25
480.54	2.28	2.28
518.54	2.85	2.85
554.52	3.44	3.44
474.58	2.58	2.58
468.53	2.42	2.42
490.49	2.39	2.39
548.59	3.99	3.99
550.56	3.18	3.18
490.49	2.39	2.39
556.64	4.02	4.02
445.50	2.00	2.00
461.53	1.84	1.84
537.06	3.86	3.86
510.57	2.14	2.14
514.63	3.21	3.21
500.55	2.85	2.85
468.53	2.42	2.42
444.47	1.70	1.70
479.52	1.98	1.98
506.94	2.90	2.90
539.59	3.16	3.16
488.95	2.76	2.76
478.53	2.09	2.09
549.65	3.79	3.79
482.56	2.88	2.88
446.53	1.92	1.92
504.51	3.04	3.04
528.56	2.47	2.47
515.57	2.68	2.68
522.50	3.13	3.13
523.57	2.00	2.00
460.54	2.17	2.17
500.51	1.82	1.82
479.52	1.98	1.98
544.56	4.69	4.69
482.56	2.71	2.71
507.57	2.63	2.63
500.55	2.90	2.90
512.56	2.99	2.99
551.58	4.42	4.42
486.52	2.51	2.51
460.54	2.17	2.17
488.95	2.76	2.76
540.62	3.77	3.77
479.52	1.98	1.98