Basic Information
ID: ALA3889180
Type: Binding
Description: Binding Competition Assay: The ability of compounds of the invention to inhibit the binding of the NK-3 receptor selective antagonist 3H-SB222200 was assessed by an in vitro radioligand binding assay. Membranes were prepared from Chinese hamster ovary recombinant cells stably expressing the human NK3 receptor. The membranes were incubated with 5 nM 3H-SB222200(ARC) in a HEPES 25 mM/NaCl 0.1M/CaCl2 1 mM/MgCl2 5 Mm/BSA 0.5%/Saponin 10 μg/ml buffer at pH 7.4 and various concentrations of compounds of the invention. The amount of 3H-SB222200 bound to the receptor was determined after filtration by the quantification of membrane associated radioactivity using the TopCount-NXT reader (Packard).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3889180
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 415.52 | 4.59 | 4.59 |
| 421.55 | 4.66 | 4.66 |
| 407.52 | 4.09 | 4.09 |
| 433.54 | 5.00 | 5.00 |
| 421.55 | 4.66 | 4.66 |
| 406.47 | 3.58 | 3.58 |
| 461.49 | 4.80 | 4.80 |
| 419.51 | 4.44 | 4.44 |
| 435.58 | 4.91 | 4.91 |
| 413.51 | 4.37 | 4.37 |
| 401.50 | 4.03 | 4.03 |
| 419.54 | 4.43 | 4.43 |
| 415.52 | 4.59 | 4.59 |
| 409.49 | 4.53 | 4.53 |
| 405.48 | 4.19 | 4.19 |
| 427.53 | 4.93 | 4.93 |
| 415.52 | 4.59 | 4.59 |
| 418.53 | 3.93 | 3.93 |
| 435.58 | 4.96 | 4.96 |
| 433.56 | 4.99 | 4.99 |
| 450.59 | 4.87 | 4.87 |
| 435.58 | 4.91 | 4.91 |
| 431.52 | 4.76 | 4.76 |
| 399.45 | 4.13 | 4.13 |
| 435.58 | 4.96 | 4.96 |
| 429.55 | 4.85 | 4.85 |
| 399.45 | 4.13 | 4.13 |
| 417.49 | 3.99 | 3.99 |
| 450.59 | 4.41 | 4.41 |