Basic Information
ID: ALA3889238
Type: Binding
Description: Radioligand Binding Assay: human or rat P2X7-1321 N1 cells were collected and frozen @−80° C. On the day of the experiment, cell membrane preparations were made according to standard published methods. The total assay volume was 100 μl: 10 μl compound (10×)+(b) 40 μl tracer (2.5×)+50 μl membrane (2×). The tracer used for the assay was tritiated A-804598. The compound can be prepared as described in the literature. (Donnelly-Roberts, D. Neuropharmacology 2009, 56 (1), 223-229.) Compounds, tracer and membranes were incubated for 1 hour @ 4° C. The assay was terminated by filtration (GF/B filters pre-soaked with 0.3% PEI) and washed with washing buffer (Tris-HCl 50 mM).
Activity Charts
Bioactivity
Activity Types for Assay ALA3889238
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 410.79 | 3.25 | 3.25 |
| 440.79 | 3.63 | 3.63 |
| 420.82 | 4.35 | 4.35 |
| 388.35 | 2.58 | 2.58 |
| 358.76 | 2.05 | 2.05 |
| 422.80 | 3.32 | 3.32 |
| 437.82 | 5.10 | 5.10 |
| 379.32 | 2.95 | 2.95 |
| 391.33 | 3.02 | 3.02 |
| 479.85 | 3.64 | 3.64 |
| 438.81 | 4.84 | 4.84 |
| 372.79 | 2.61 | 2.61 |
| 386.28 | 4.69 | 4.69 |
| 406.75 | 3.36 | 3.36 |
| 439.80 | 4.06 | 4.06 |
| 420.78 | 3.92 | 3.92 |
| 406.34 | 2.97 | 2.97 |
| 421.81 | 4.09 | 4.09 |
| 389.25 | 3.12 | 3.12 |
| 406.25 | 3.70 | 3.70 |
| 405.77 | 3.96 | 3.96 |
| 422.80 | 3.49 | 3.49 |
| 406.25 | 3.70 | 3.70 |
| 419.83 | 5.30 | 5.30 |
| 439.80 | 4.23 | 4.23 |
| 437.78 | 4.66 | 4.66 |
| 445.31 | 3.88 | 3.88 |
| 424.33 | 2.94 | 2.94 |
| 437.85 | 4.08 | 4.08 |
| 403.27 | 3.43 | 3.43 |
| 424.81 | 3.81 | 3.81 |
| 439.80 | 4.23 | 4.23 |
| 422.80 | 3.49 | 3.49 |
| 437.82 | 5.44 | 5.44 |
| 440.79 | 3.46 | 3.46 |
| 375.22 | 2.56 | 2.56 |
| 447.89 | 5.87 | 5.87 |
| 439.80 | 4.23 | 4.23 |
| 409.80 | 4.06 | 4.06 |
| 406.80 | 4.14 | 4.14 |
| 372.25 | 4.38 | 4.38 |
| 410.79 | 3.42 | 3.42 |
| 439.80 | 4.23 | 4.23 |
| 407.24 | 3.26 | 3.26 |
| 437.82 | 5.44 | 5.44 |
| 395.77 | 3.47 | 3.47 |
| 478.86 | 4.24 | 4.24 |
| 422.80 | 3.49 | 3.49 |
| 421.81 | 4.09 | 4.09 |
| 439.80 | 4.23 | 4.23 |
| 392.32 | 2.41 | 2.41 |
| 420.37 | 3.11 | 3.11 |
| 406.25 | 3.70 | 3.70 |
| 402.29 | 4.04 | 4.04 |
| 438.81 | 4.84 | 4.84 |
| 440.25 | 5.40 | 5.40 |
| 437.82 | 5.44 | 5.44 |
| 433.82 | 4.91 | 4.91 |
| 425.32 | 2.34 | 2.34 |
| 477.87 | 3.77 | 3.77 |
| 410.79 | 3.42 | 3.42 |
| 406.25 | 3.87 | 3.87 |
| 375.22 | 2.56 | 2.56 |
| 449.86 | 4.71 | 4.71 |
| 394.33 | 2.74 | 2.74 |
| 435.84 | 4.40 | 4.40 |
| 454.82 | 3.94 | 3.94 |
| 436.83 | 3.80 | 3.80 |
| 424.79 | 4.28 | 4.28 |
| 424.33 | 2.94 | 2.94 |
| 446.29 | 3.27 | 3.27 |
| 407.78 | 3.53 | 3.53 |
| 434.85 | 5.09 | 5.09 |
| 400.31 | 4.94 | 4.94 |
| 392.22 | 3.31 | 3.31 |
| 489.89 | 3.91 | 3.91 |
| 402.38 | 3.14 | 3.14 |
| 387.27 | 4.33 | 4.33 |
| 406.80 | 4.34 | 4.34 |
| 422.80 | 3.32 | 3.32 |
| 406.34 | 2.80 | 2.80 |
| 411.77 | 3.69 | 3.69 |
| 408.77 | 2.93 | 2.93 |
| 433.86 | 5.48 | 5.48 |
| 407.24 | 3.26 | 3.26 |
| 435.84 | 4.40 | 4.40 |
| 425.77 | 3.67 | 3.67 |
| 375.22 | 2.56 | 2.56 |
| 387.27 | 3.99 | 3.99 |
| 425.23 | 3.23 | 3.23 |
| 436.82 | 4.15 | 4.15 |
| 373.24 | 3.77 | 3.77 |
| 407.24 | 3.09 | 3.09 |
| 421.81 | 3.92 | 3.92 |
| 425.77 | 3.67 | 3.67 |
| 482.85 | 4.95 | 4.95 |
| 435.84 | 4.40 | 4.40 |
| 420.82 | 4.53 | 4.53 |
| 439.80 | 4.06 | 4.06 |
| 405.77 | 3.96 | 3.96 |
| 437.82 | 5.10 | 5.10 |
| 408.77 | 2.93 | 2.93 |
| 387.27 | 3.99 | 3.99 |
| 406.80 | 4.34 | 4.34 |
| 421.81 | 3.92 | 3.92 |
| 435.84 | 4.40 | 4.40 |
| 402.38 | 3.14 | 3.14 |
| 406.80 | 4.14 | 4.14 |
| 439.80 | 4.23 | 4.23 |
| 422.80 | 3.49 | 3.49 |
| 436.84 | 6.19 | 6.19 |
| 423.25 | 4.61 | 4.61 |
| 420.82 | 4.53 | 4.53 |
| 358.76 | 2.05 | 2.05 |
| 426.76 | 3.07 | 3.07 |
| 438.81 | 4.65 | 4.65 |
| 435.84 | 4.40 | 4.40 |
| 390.25 | 4.72 | 4.72 |
| 452.84 | 5.40 | 5.40 |
| 407.78 | 3.74 | 3.74 |
| 468.84 | 4.49 | 4.49 |
| 420.82 | 4.70 | 4.70 |
| 425.23 | 3.23 | 3.23 |
| 451.84 | 4.10 | 4.10 |
| 406.25 | 3.70 | 3.70 |
| 419.83 | 5.05 | 5.05 |
| 436.83 | 3.80 | 3.80 |
| 418.39 | 4.49 | 4.49 |
| 408.77 | 3.85 | 3.85 |
| 422.80 | 3.49 | 3.49 |
| 407.78 | 3.53 | 3.53 |
| 463.89 | 4.24 | 4.24 |
| 465.86 | 4.49 | 4.49 |
| 404.37 | 3.93 | 3.93 |
| 434.85 | 5.26 | 5.26 |
| 373.24 | 3.98 | 3.98 |
| 439.80 | 4.06 | 4.06 |
| 444.32 | 4.48 | 4.48 |
| 440.79 | 3.63 | 3.63 |
| 440.79 | 3.46 | 3.46 |
| 425.23 | 3.23 | 3.23 |
| 452.84 | 5.23 | 5.23 |
| 456.83 | 3.87 | 3.87 |
| 421.37 | 4.93 | 4.93 |
| 420.82 | 4.35 | 4.35 |
| 436.80 | 5.27 | 5.27 |
| 449.86 | 4.66 | 4.66 |
| 451.84 | 4.10 | 4.10 |
| 422.80 | 3.49 | 3.49 |
| 405.36 | 3.58 | 3.58 |
| 449.86 | 4.73 | 4.73 |
| 424.79 | 4.48 | 4.48 |
| 468.87 | 3.54 | 3.54 |
| 420.82 | 4.70 | 4.70 |
| 473.80 | 5.76 | 5.76 |
| 407.24 | 3.09 | 3.09 |
| 372.25 | 4.58 | 4.58 |
| 405.81 | 4.74 | 4.74 |
| 439.80 | 4.06 | 4.06 |
| 484.87 | 4.52 | 4.52 |
| 388.26 | 3.56 | 3.56 |
| 420.82 | 4.70 | 4.70 |
| 438.81 | 4.84 | 4.84 |
| 389.25 | 3.12 | 3.12 |
| 373.24 | 3.77 | 3.77 |
| 422.36 | 4.32 | 4.32 |
| 438.81 | 4.84 | 4.84 |
| 437.81 | 3.54 | 3.54 |
| 355.35 | 2.45 | 2.45 |
| 435.83 | 4.28 | 4.28 |
| 402.29 | 4.04 | 4.04 |
| 419.79 | 4.52 | 4.52 |
| 421.81 | 3.75 | 3.75 |
| 420.82 | 4.35 | 4.35 |
| 372.79 | 2.61 | 2.61 |
| 386.28 | 4.59 | 4.59 |
| 420.78 | 3.92 | 3.92 |
| 422.80 | 3.32 | 3.32 |
| 388.35 | 2.58 | 2.58 |
| 392.32 | 2.41 | 2.41 |
| 410.79 | 2.87 | 2.87 |
| 423.80 | 5.09 | 5.09 |
| 362.22 | 3.10 | 3.10 |
| 434.85 | 5.09 | 5.09 |
| 396.76 | 2.86 | 2.86 |
| 477.87 | 4.85 | 4.85 |
| 425.23 | 3.23 | 3.23 |
| 406.25 | 3.70 | 3.70 |
| 419.29 | 4.86 | 4.86 |
| 435.84 | 4.48 | 4.48 |
| 479.89 | 4.88 | 4.88 |
| 406.80 | 4.26 | 4.26 |
| 386.28 | 4.59 | 4.59 |
| 450.85 | 4.11 | 4.11 |
| 407.78 | 3.74 | 3.74 |
| 420.37 | 3.11 | 3.11 |
| 457.35 | 5.25 | 5.25 |
| 424.79 | 4.48 | 4.48 |
| 424.33 | 3.11 | 3.11 |
| 375.22 | 2.56 | 2.56 |
| 424.81 | 3.43 | 3.43 |
| 405.81 | 4.95 | 4.95 |
| 404.37 | 4.18 | 4.18 |
| 438.81 | 4.84 | 4.84 |
| 402.29 | 4.04 | 4.04 |
| 420.37 | 3.28 | 3.28 |
| 422.80 | 3.49 | 3.49 |
| 422.80 | 3.49 | 3.49 |
| 440.79 | 3.63 | 3.63 |
| 439.80 | 4.23 | 4.23 |
| 436.83 | 3.80 | 3.80 |
| 435.84 | 4.40 | 4.40 |
| 424.81 | 3.81 | 3.81 |
| 439.80 | 4.06 | 4.06 |
| 424.81 | 3.81 | 3.81 |
| 438.84 | 3.42 | 3.42 |
| 406.34 | 2.97 | 2.97 |
| 450.85 | 4.05 | 4.05 |
| 454.82 | 3.94 | 3.94 |
| 406.25 | 3.70 | 3.70 |
| 435.84 | 4.40 | 4.40 |
| 410.79 | 3.42 | 3.42 |
| 452.84 | 5.23 | 5.23 |
| 439.80 | 4.44 | 4.44 |
| 421.81 | 3.92 | 3.92 |
| 465.86 | 4.49 | 4.49 |
| 402.38 | 3.14 | 3.14 |
| 404.37 | 4.18 | 4.18 |