Basic Information
ID: ALA3889238
Type: Binding
Description: Radioligand Binding Assay: human or rat P2X7-1321 N1 cells were collected and frozen @−80° C. On the day of the experiment, cell membrane preparations were made according to standard published methods. The total assay volume was 100 μl: 10 μl compound (10×)+(b) 40 μl tracer (2.5×)+50 μl membrane (2×). The tracer used for the assay was tritiated A-804598. The compound can be prepared as described in the literature. (Donnelly-Roberts, D. Neuropharmacology 2009, 56 (1), 223-229.) Compounds, tracer and membranes were incubated for 1 hour @ 4° C. The assay was terminated by filtration (GF/B filters pre-soaked with 0.3% PEI) and washed with washing buffer (Tris-HCl 50 mM).
Format: BAO_0000019
Target: P2X purinoceptor 7(ALA4805)
Document: ALA3887003
BindingDB Source: 8504
Activity Charts
Bioactivity
Activity Types for Assay ALA3889238
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 420.82 | 4.35 | 4.35 |
| 437.82 | 5.10 | 5.10 |
| 379.32 | 2.95 | 2.95 |
| 479.85 | 3.64 | 3.64 |
| 438.81 | 4.84 | 4.84 |
| 406.75 | 3.36 | 3.36 |
| 420.78 | 3.92 | 3.92 |
| 421.81 | 4.09 | 4.09 |
| 405.77 | 3.96 | 3.96 |
| 419.83 | 5.30 | 5.30 |
| 437.78 | 4.66 | 4.66 |
| 445.31 | 3.88 | 3.88 |
| 439.80 | 4.23 | 4.23 |
| 422.80 | 3.49 | 3.49 |
| 437.82 | 5.44 | 5.44 |
| 375.22 | 2.56 | 2.56 |
| 439.80 | 4.23 | 4.23 |
| 406.80 | 4.14 | 4.14 |
| 372.25 | 4.38 | 4.38 |
| 437.82 | 5.44 | 5.44 |
| 395.77 | 3.47 | 3.47 |
| 478.86 | 4.24 | 4.24 |
| 422.80 | 3.49 | 3.49 |
| 421.81 | 4.09 | 4.09 |
| 439.80 | 4.23 | 4.23 |
| 392.32 | 2.41 | 2.41 |
| 438.81 | 4.84 | 4.84 |
| 440.25 | 5.40 | 5.40 |
| 437.82 | 5.44 | 5.44 |
| 433.82 | 4.91 | 4.91 |
| 477.87 | 3.77 | 3.77 |
| 410.79 | 3.42 | 3.42 |
| 446.29 | 3.27 | 3.27 |
| 407.78 | 3.53 | 3.53 |
| 489.89 | 3.91 | 3.91 |
| 406.80 | 4.34 | 4.34 |
| 408.77 | 2.93 | 2.93 |
| 433.86 | 5.48 | 5.48 |
| 387.27 | 3.99 | 3.99 |
| 373.24 | 3.77 | 3.77 |
| 421.81 | 3.92 | 3.92 |
| 482.85 | 4.95 | 4.95 |
| 405.77 | 3.96 | 3.96 |
| 437.82 | 5.10 | 5.10 |
| 408.77 | 2.93 | 2.93 |
| 387.27 | 3.99 | 3.99 |
| 406.80 | 4.34 | 4.34 |
| 421.81 | 3.92 | 3.92 |
| 406.80 | 4.14 | 4.14 |
| 422.80 | 3.49 | 3.49 |
| 426.76 | 3.07 | 3.07 |
| 438.81 | 4.65 | 4.65 |
| 390.25 | 4.72 | 4.72 |
| 407.78 | 3.74 | 3.74 |
| 419.83 | 5.05 | 5.05 |
| 408.77 | 3.85 | 3.85 |
| 422.80 | 3.49 | 3.49 |
| 407.78 | 3.53 | 3.53 |
| 463.89 | 4.24 | 4.24 |
| 373.24 | 3.98 | 3.98 |
| 444.32 | 4.48 | 4.48 |
| 421.37 | 4.93 | 4.93 |
| 420.82 | 4.35 | 4.35 |
| 436.80 | 5.27 | 5.27 |
| 405.36 | 3.58 | 3.58 |
| 449.86 | 4.73 | 4.73 |
| 424.79 | 4.48 | 4.48 |
| 420.82 | 4.70 | 4.70 |
| 473.80 | 5.76 | 5.76 |
| 372.25 | 4.58 | 4.58 |
| 405.81 | 4.74 | 4.74 |
| 484.87 | 4.52 | 4.52 |
| 420.82 | 4.70 | 4.70 |
| 373.24 | 3.77 | 3.77 |
| 438.81 | 4.84 | 4.84 |
| 435.83 | 4.28 | 4.28 |
| 419.79 | 4.52 | 4.52 |
| 421.81 | 3.75 | 3.75 |
| 420.82 | 4.35 | 4.35 |
| 386.28 | 4.59 | 4.59 |
| 420.78 | 3.92 | 3.92 |
| 392.32 | 2.41 | 2.41 |
| 423.80 | 5.09 | 5.09 |
| 362.22 | 3.10 | 3.10 |
| 396.76 | 2.86 | 2.86 |
| 477.87 | 4.85 | 4.85 |
| 435.84 | 4.48 | 4.48 |
| 406.80 | 4.26 | 4.26 |
| 386.28 | 4.59 | 4.59 |
| 407.78 | 3.74 | 3.74 |
| 457.35 | 5.25 | 5.25 |
| 424.79 | 4.48 | 4.48 |
| 375.22 | 2.56 | 2.56 |
| 405.81 | 4.95 | 4.95 |
| 404.37 | 4.18 | 4.18 |
| 438.81 | 4.84 | 4.84 |
| 422.80 | 3.49 | 3.49 |
| 422.80 | 3.49 | 3.49 |
| 421.81 | 3.92 | 3.92 |
| 404.37 | 4.18 | 4.18 |