Basic Information
ID: ALA3889239
Type: Binding
Description: Radioligand Binding Assay: human or rat P2X7-1321 N1 cells were collected and frozen @−80° C. On the day of the experiment, cell membrane preparations were made according to standard published methods. The total assay volume was 100 μl: 10 μl compound (10×)+(b) 40 μl tracer (2.5×)+50 μl membrane (2×). The tracer used for the assay was tritiated A-804598. The compound can be prepared as described in the literature. (Donnelly-Roberts, D. Neuropharmacology 2009, 56 (1), 223-229.) Compounds, tracer and membranes were incubated for 1 hour @ 4° C. The assay was terminated by filtration (GF/B filters pre-soaked with 0.3% PEI) and washed with washing buffer (Tris-HCl 50 mM).
Activity Charts
Bioactivity
Activity Types for Assay ALA3889239
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 438.81 | 4.84 | 4.84 |
| 419.83 | 5.30 | 5.30 |
| 422.80 | 3.49 | 3.49 |
| 440.79 | 3.46 | 3.46 |
| 422.80 | 3.49 | 3.49 |
| 420.37 | 3.11 | 3.11 |
| 424.33 | 2.94 | 2.94 |
| 404.37 | 4.18 | 4.18 |
| 408.77 | 2.93 | 2.93 |
| 422.80 | 3.49 | 3.49 |
| 426.76 | 3.07 | 3.07 |
| 456.83 | 3.87 | 3.87 |
| 468.87 | 3.54 | 3.54 |
| 424.81 | 3.43 | 3.43 |
| 405.81 | 4.95 | 4.95 |
| 422.80 | 3.49 | 3.49 |
| 422.80 | 3.49 | 3.49 |
| 440.79 | 3.63 | 3.63 |
| 436.83 | 3.80 | 3.80 |
| 410.79 | 3.42 | 3.42 |
| 421.81 | 3.92 | 3.92 |
| 404.37 | 4.18 | 4.18 |