Basic Information
ID: ALA3889300
Type: Binding
Description: Cellular Firefly Luciferase Assay (AP-1): Curcumin is a known inhibitor of the AP-1 activation cascade. Therefore, modification of the structure of curcumin could lead to analogs with enhanced activity. The library consisting of three series of curcumin analogs were used to examine the role of the enone functionality in aryl systems where the spacer is 7-carbons (as in curcumin), 5-carbons or 3-carbons in length. In addition, the importance of aryl ring substituents was assessed. The AP-1 activities of curcumin and analogs were determined by a cellular firefly luciferase assay. This assay utilized a commercially available cell line (Panomics 293-luc cellular assay) developed for screening inhibitors of AP-1. This cell line is stably transfected with a luciferase reporter controlled by an AP-1 dependent promoter. The cell is stimulated with phorbol ester which activates AP-1. AP-1 then binds to one of three promoter regions on the cells DNA leading to the production of a luciferase enzyme. Luciferin is added to the cell lysates and the luciferase enzyme catalyzes a cleavage of luciferin leading to the emission of light.
Activity Charts
Bioactivity
Activity Types for Assay ALA3889300
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 366.41 | 4.34 | 4.34 |
| 368.39 | 3.85 | 3.85 |
| 266.30 | 3.39 | 3.39 |
| 410.42 | 3.85 | 3.85 |
| 354.40 | 4.02 | 4.02 |
| 382.41 | 4.24 | 4.24 |
| 236.27 | 2.77 | 2.77 |
| 236.27 | 2.77 | 2.77 |
| 458.51 | 5.47 | 5.47 |