Basic Information
ID: ALA3889301
Type: Binding
Description: Cellular Firefly Luciferase Assay (NFkB): The NF-kB activities of curcumin and analogs were determined by a cellular firefly luciferase assay. This assay utilized a commercially available cell line (Panomics 293T-luc cellular assay) developed for screening inhibitors of NF-kB. This cell line is stably transfected with a luciferase reporter controlled by an NF-kB dependent promoter. The cell is stimulated with tumor necrosis factor alpha (TNFalpha) which activates NF-kB. NF-kB then binds to one of six promoter regions on the cell's DNA leading to the production of a luciferase enzyme. Luciferin is added to the cell lysates and the luciferase enzyme catalyzes a cleavage of luciferin leading to the emission of light.
Format: BAO_0000019
Target: Nuclear factor NF-kappa-B p105 subunit(ALA3251)
Document: ALA3886177
BindingDB Source: 7369
Activity Charts
Bioactivity
Activity Types for Assay ALA3889301
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 366.41 | 4.34 | 4.34 |
| 368.39 | 3.85 | 3.85 |
| 370.29 | 6.02 | 6.02 |
| 266.30 | 3.39 | 3.39 |
| 410.42 | 3.85 | 3.85 |
| 354.40 | 4.02 | 4.02 |
| 382.41 | 4.24 | 4.24 |
| 236.27 | 2.77 | 2.77 |
| 236.27 | 2.77 | 2.77 |
| 368.39 | 2.85 | 2.85 |
| 458.51 | 5.47 | 5.47 |