Basic Information
ID: ALA3889302
Type: Binding
Description: Inhibition Assay: TRPV3 cells were induced 20-48 hours, removed from growth plates, and replated at low density (to attain good single-cell physical separation) on glass coverslips for measurement. In some cases, cells were grown in low density overnight on glass coverslips. Patch clamp recordings were made in the whole-cell mode with a holding potential of -40 mV. Every 5 seconds, a voltage ramp was applied from -120 to +100 mV, 400 ms in duration. Currents elicited were quantified at -80 mV and +80 mV. The internal solution consisted of 140 mM cesium aspartate, 10 mM EGTA, 2.27 mM MgCl2, 1.91 mM CaCl2 and 10 mM HEPES, pH to 7.2 with KOH; with 50 nM calculated free Ca2+. External solution was Ringer's solution described above. Upon addition of 2-APB or upon heating of the extraceullar solution as described above, TRPV3 current was induced only in TRPV3-expressing cells and not in parental HEK293 TREx cells.
Organism: Homo sapiens
Cell type: TREx
Activity Charts
Bioactivity
Activity Types for Assay ALA3889302
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 326.47 | 4.08 | 4.08 |
| 366.41 | 4.48 | 4.48 |
| 323.42 | 3.33 | 3.33 |
| 298.41 | 3.46 | 3.46 |
| 312.44 | 3.77 | 3.77 |
| 332.86 | 4.11 | 4.11 |
| 323.42 | 3.33 | 3.33 |
| 323.42 | 3.33 | 3.33 |
| 394.46 | 5.04 | 5.04 |
| 377.31 | 4.22 | 4.22 |
| 366.41 | 4.48 | 4.48 |
| 352.38 | 4.17 | 4.17 |
| 332.86 | 4.11 | 4.11 |
| 400.85 | 5.13 | 5.13 |
| 384.40 | 4.62 | 4.62 |
| 367.30 | 4.77 | 4.77 |
| 390.89 | 3.90 | 3.90 |
| 326.47 | 4.08 | 4.08 |
| 318.83 | 3.81 | 3.81 |
| 348.86 | 3.82 | 3.82 |