Basic Information
ID: ALA4353689
Type: Binding
Description: Inhibition of N-terminal GST-tagged recombinant human KDM4B (1 to 500 residues) expressed in baculovirus infected Sf9 insect cells assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 20 mins by AlphaScreen assay
Format: BAO_0000219
Organism: Homo sapiens
Cell type: Sf9
Target: Lysine-specific demethylase 4B(ALA3313832)
Document: ALA4350996
Inhibition of N-terminal GST-tagged recombinant human KDM4B (1 to 500 residues) expressed in baculovirus infected Sf9 insect cells assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 20 mins by AlphaScreen assay: 1
Activity Charts
Bioactivity
Activity Types for Assay ALA4353689
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 454.58 | 3.64 | 3.64 |
| 478.56 | 3.38 | 3.38 |
| 471.61 | 3.03 | 3.03 |
| 513.65 | 2.80 | 2.80 |
| 352.44 | 2.34 | 2.34 |
| 418.48 | 3.07 | 3.07 |
| 426.52 | 3.03 | 3.03 |
| 338.42 | 2.09 | 2.09 |
| 428.50 | 2.57 | 2.57 |
| 513.65 | 2.80 | 2.80 |
| 472.59 | 3.96 | 3.96 |
| 442.57 | 4.05 | 4.05 |
| 310.36 | 1.31 | 1.31 |
| 390.45 | 1.62 | 1.62 |
| 551.62 | 4.54 | 4.54 |
| 456.59 | 4.22 | 4.22 |
| 483.58 | 3.05 | 3.05 |
| 364.45 | 2.48 | 2.48 |
| 364.45 | 2.63 | 2.63 |
| 350.43 | 2.09 | 2.09 |
| 430.51 | 2.93 | 2.93 |
| 499.62 | 2.76 | 2.76 |
| 581.64 | 3.82 | 3.82 |
| 478.56 | 3.38 | 3.38 |
| 477.57 | 3.99 | 3.99 |
| 324.39 | 1.70 | 1.70 |
| 477.57 | 3.99 | 3.99 |
| 477.57 | 3.99 | 3.99 |
| 477.57 | 3.99 | 3.99 |
| 448.96 | 3.75 | 3.75 |
| 471.61 | 3.03 | 3.03 |
| 477.57 | 3.99 | 3.99 |
| 483.62 | 3.52 | 3.52 |