Selectivity index, Ratio of Kd for wild type human N-terminal TEV cleavage site-fused-His6-tagged JAK2 JH1 domain (840 to 1132 residues) expressed in ...

ID: ALA4359665

Type: Unassigned

Description: Selectivity index, Ratio of Kd for wild type human N-terminal TEV cleavage site-fused-His6-tagged JAK2 JH1 domain (840 to 1132 residues) expressed in baculovirus infected sf9 insect cells measured for 1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay to Kd for binding affinity to human C-terminal thrombin cleavage site-fused/ 6xHis-tagged JAK2 JH2 pseudokinase domain V617F mutant (536 to 812 residues) expressed in baculovirus infected sf9 insect cells measured for 1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay

Format: BAO_0000219

Organism: Homo sapiens

Target:  Unchecked(ALA612545)

Document:  ALA4354882

Selectivity index, Ratio of Kd for wild type human N-terminal TEV cleavage site-fused-His6-tagged JAK2 JH1 domain (840 to 1132 residues) expressed in baculovirus infected sf9 insect cells measured for 1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay to Kd for binding affinity to human C-terminal thrombin cleavage site-fused/ 6xHis-tagged JAK2 JH2 pseudokinase domain V617F mutant (536 to 812 residues) expressed in baculovirus infected sf9 insect cells measured for 1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay: 1