Basic Information
ID: ALA4392309
Type: Binding
Description: Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as reduction in DHFR steady state activity at 500 uM using [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control
Format: BAO_0000019
Organism: Cryptosporidium hominis
Target: Bifunctional dihydrofolate reductase-thymidylate synthase(ALA4296761)
Document: ALA4390681
Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as reduction in DHFR steady state activity at 500 uM using [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control: 1
Activity Charts
Bioactivity
Activity Types for Assay ALA4392309
Inhibition
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 292.13 | 3.41 | 3.41 |
| 162.15 | 1.04 | 1.04 |
| 326.58 | 4.06 | 4.06 |
| 228.20 | -1.82 | -1.82 |
| 373.58 | 3.90 | 3.90 |
| 361.02 | 4.71 | 4.71 |
| 282.13 | 3.95 | 3.95 |
| 271.30 | 1.08 | 1.08 |
| 373.41 | 2.78 | 2.78 |
| 282.13 | 3.95 | 3.95 |
| 428.13 | 5.44 | 5.44 |
| 268.23 | 1.08 | 1.08 |
| 339.18 | 2.56 | 2.56 |
| 310.12 | 3.55 | 3.55 |
| 220.15 | 1.21 | 1.21 |
| 308.13 | 3.11 | 3.11 |
| 456.37 | 5.52 | 5.52 |
| 270.29 | 2.00 | 2.00 |
| 324.20 | 3.70 | 3.70 |
| 262.70 | 2.88 | 2.88 |
| 204.27 | 2.45 | 2.45 |
| 259.33 | 3.24 | 3.24 |
| 276.21 | 4.04 | 4.04 |
| 344.57 | 4.20 | 4.20 |
| 228.25 | 2.23 | 2.23 |
| 533.92 | 5.16 | 5.16 |
| 279.27 | 1.28 | 1.28 |
| 366.37 | 0.14 | 0.14 |
| 448.10 | 4.74 | 4.74 |
| 296.15 | 4.26 | 4.26 |
| 242.28 | 2.10 | 2.10 |
| 223.20 | 0.94 | 0.94 |
| 269.33 | 2.62 | 2.62 |