Basic Information
ID: ALA4392315
Type: Binding
Description: Mixed noncompetitive inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as substrate Km at 250 uM using varying levels of [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by Lineweaver-Burk plot analysis (Rvb = 1.2 +/- 0.2 uM)
Organism: Cryptosporidium hominis
Activity Charts
Bioactivity
Activity Types for Assay ALA4392315
Km
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 456.37 | 5.52 | 5.52 |