Basic Information
ID: ALA4392317
Type: Binding
Description: Mixed noncompetitive inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as Kobs at 250 uM using varying levels of [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by Lineweaver-Burk plot analysis (Rvb = 1.98 +/- 0.09 /s)
Format: BAO_0000019
Organism: Cryptosporidium hominis
Target: Bifunctional dihydrofolate reductase-thymidylate synthase(ALA4296761)
Document: ALA4390681
Mixed noncompetitive inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as Kobs at 250 uM using varying levels of [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by Lineweaver-Burk plot analysis (Rvb = 1.98 +/- 0.09 /s): 1
Activity Charts
Bioactivity
Activity Types for Assay ALA4392317
K
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 456.37 | 5.52 | 5.52 |