Basic Information
ID: ALA4392319
Type: Binding
Description: Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as reduction in DHFR steady state activity at 500 uM using [3',5',7,9-3H]-H2folate as substrate preincubated for 1 hr to 72 hrs before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control
Format: BAO_0000019
Organism: Cryptosporidium hominis
Target: Bifunctional dihydrofolate reductase-thymidylate synthase(ALA4296761)
Document: ALA4390681
Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as reduction in DHFR steady state activity at 500 uM using [3',5',7,9-3H]-H2folate as substrate preincubated for 1 hr to 72 hrs before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control: 1
Activity Charts
Bioactivity
Activity Types for Assay ALA4392319
Inhibition
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 324.20 | 3.70 | 3.70 |
| 259.33 | 3.24 | 3.24 |