Affinity probes for the avermectin binding proteins.
Basic Information
ID: ALA1126310
Journal: J Med Chem
Title: Affinity probes for the avermectin binding proteins.
Authors: Meinke PT, Rohrer SP, Hayes EC, Schaeffer JM, Fisher MH, Mrozik H.
Abstract: The design and synthesis of a series of avermectin affinity probes used in the identification and purification of the avermectin binding proteins is described. These modified avermectins fall into two design classes: ligands to covalently modify specific avermectin binding proteins [an 125I-labeled aryl azide photoprobe (15) and a tritiated aziridine analog (6)] and ligands for affinity chromatography applications [three biotinylated compounds (10, 12, and 13) and one resin-bound derivative (9)]. The binding affinities of these compounds for the Caenorhabditis elegans avermectin binding protein is presented as well as their biological activities against C. elegans and Artemia salina.
CiteXplore: 1433197
DOI: 10.1021/jm00099a015
Patent ID: ┄