Document Report Card

ID: ALA1127649

Journal: J Med Chem

Title: Protein kinase C. Modeling of the binding site and prediction of binding constants.

Authors: Wang S, Milne GW, Nicklaus MC, Marquez VE, Lee J, Blumberg PM.

Abstract: A detailed examination of the mode of binding of phorbol esters to protein kinase C led to a model of the phorbol binding site in the enzyme. The efficacy with which various synthetic diacylglycerol analogs and ribonolactones are able to bind to this site was determined by means of semiempirical quantum mechanical calculations using PM3, and an estimate of the binding energy was made in each case. Sixteen synthetic analogs of 1,2-diacylglycerol and two natural products were studied, and their calculated energies of binding to this model were correlated with the measured Ki values. The binding energies calculated for this receptor model, together with solubility and entropy considerations, allow prediction through regressive fit of free energies of binding which correlate very well with the measured binding constants.

CiteXplore: 8176711

DOI: 10.1021/jm00035a013