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ID: ALA1131944

Journal: Bioorg Med Chem Lett

Title: Phospholipase A2-like catalytic antibody.

Authors: Isomura S, Ito K, Haruna M.

Abstract: The phospholipase A2-like catalytic antibody 13C2-1F6 was elicited against the hapten 1 as the transition state analog for the hydrolysis of the C2 ester in the phospholipid. The Michaelis-Menten kinetics for the hydrolysis of the phospholipid 2 by 13C2-1F6 afforded a kcat of 1.0 x 10(-2) min(-1) and aKm of 71 microM. This antibody hydrolyzes the C2 ester in (R)-2, regio- and enantioselectively.

CiteXplore: 10098662

DOI: 10.1016/s0960-894x(99)00035-9