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ID: ALA1135102

Journal: Bioorg Med Chem Lett

Title: 2-Amino-7-deazaadenine forms stable base pairs with cytosine and thymine.

Authors: Okamoto A, Tanaka K, Saito I.

Abstract: 2-Amino-7-deazaadenine ((AD)A) was incorporated into oligodeoxynucleotides (ODN) and their base-pairing properties with natural nucleobases were investigated. In melting temperature (T(m)) experiments, the duplex containing an (AD)A/C base pair showed a high stability comparable to that containing (AD)A/T base pair. Destabilization of the duplex usually observed for existing degenerate bases was not observed. However, the incorporation efficiency of dCTP was only 1.8% for TTP in single-nucleotide insertion reactions using polymerase.

CiteXplore: 11738582

DOI: 10.1016/s0960-894x(01)00685-0