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ID: ALA1136117

Journal: Bioorg Med Chem Lett

Title: Identification of oxidation product of arbutin in mushroom tyrosinase assay system.

Authors: Nihei Ki, Kubo I.

Abstract: In order to elucidate whitening mechanisms of arbutin (hydroquinone-O-beta-D-glucopyranoside), its effects on mushroom tyrosinase were analyzed by spectrophotometric, polarographic, and HPLC experiments. It was found that as soon as catalytic amounts of L-DOPA become available as a cofactor, arbutin acts as a monophenol substrate. A significant enzymatic product was identified as 3,4-dihydroxyphenyl-O-beta-D-glucopyranoside by NMR and MS experiments.

CiteXplore: 12824045

DOI: 10.1016/s0960-894x(03)00395-0