Document Report Card

ID: ALA1137639

Journal: Antimicrob Agents Chemother

Title: Novel small-molecule inhibitors of transmissible gastroenteritis virus.

Authors: Yang CW, Yang YN, Liang PH, Chen CM, Chen WL, Chang HY, Chao YS, Lee SJ.

Abstract: We used swine testicle (ST) cells infected with transmissible gastroenteritis virus (TGEV) and an indirect immunofluorescent assay with antibodies against TGEV spike and nucleocapsid proteins to screen small-molecule compounds that inhibit TGEV replication. Analogues of initial hits were collected and subjected to a 3CL protease (3CL(pro)) inhibition assay with recombinant 3CL(pro) and a fluorogenic peptide substrate. A series of benzothiazolium compounds were found to have inhibitory activity against TGEV 3CL(pro) and to exert anti-TGEV activities in terms of viral protein and RNA replication in TGEV-infected ST cells, with consequent protection of TGEV-infected ST cells from cytopathic effect by blocking the activation of caspase-3.

CiteXplore: 17709464

DOI: 10.1128/aac.00408-07