Document Report Card

ID: ALA1137666

Journal: Antimicrob Agents Chemother

Title: Biochemical Characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase.

Authors: Fonseca F, Sarmento AC, Henriques I, Samyn B, van Beeumen J, Domingues P, Domingues MR, Saavedra MJ, Correia A.

Abstract: The carbapenem-hydrolyzing beta-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.

CiteXplore: 17875998

DOI: 10.1128/aac.00491-07