Document Report Card

ID: ALA1141274

Journal: J Nat Prod

Title: A Polysaccharide from tomato (Lycopersicon esculentum) peels affects NF-kappaB activation in LPS-stimulated J774 macrophages.

Authors: De Stefano D, Tommonaro G, Simeon V, Poli A, Nicolaus B, Carnuccio R.

Abstract: We investigated the effect of PS ( 1) on nitrite and reactive oxygen species (ROS) production in J774 macrophages stimulated with bacterial lipopolysaccharide (LPS) for 24 h. PS ( 1) inhibited in a concentration-dependent manner nitrite and ROS production as well as inducible nitric oxide synthase (iNOS) protein expression induced by LPS. Incubation of cells with PS ( 1) determined a significant decrease of nuclear factor-kappaB (NF-kappaB)/DNA binding activity which was correlated with a marked reduction of iNOS mRNA levels. These results show that PS ( 1) inhibits NF-kappaB activation and iNOS gene expression by preventing the reactive species production and suggest a role for this compound in controlling oxidative stress and/or inflammation.

CiteXplore: 17764147

DOI: 10.1021/np070168z