Document Report Card

ID: ALA1145818

Journal: J Med Chem

Title: Effect of detergent on "promiscuous" inhibitors.

Authors: Ryan AJ, Gray NM, Lowe PN, Chung CW.

Abstract: The term "promiscuous" inhibitors has been coined for compounds whose inhibition mechanism involves the interaction of aggregates of many compound molecules with the target protein, rather than the binding of individual molecules. This paper demonstrates that promiscuous inhibitors can be differentiated from classical 1:1 inhibitors by the judicious use of detergents, making it possible to configure assays that significantly reduce this undesirable mechanism of inhibition without compromising assay performance.

CiteXplore: 12877581

DOI: 10.1021/jm0340896