Document Report Card

ID: ALA1146195

Journal: Antimicrob Agents Chemother

Title: Use of novel boronic acid transition state inhibitors to probe substrate affinity in SHV-type extended-spectrum beta-lactamases.

Authors: Thomson JM, Prati F, Bethel CR, Bonomo RA.

Abstract: Boronic acid transition state inhibitors (BATSIs) with R1 side chains of cefotaxime and ceftazidime were assayed against SHV-1, SHV-2, SHV-5, D104K, and D104K G238S beta-lactamases. The D104K variant was the most susceptible to inhibition by the ceftazidime BATSI (Ki, 730+/-80 nM), while the D104K G238S variant was the most susceptible to the cefotaxime BATSI (Ki, 1.1+/-0.2 microM).

CiteXplore: 17220410

DOI: 10.1128/aac.01293-06