Document Report Card

ID: ALA1146501

Journal: J Nat Prod

Title: Microbial transformation of isosteviol and inhibitory effects on Epstein-Barr virus activation of the transformation products.

Authors: Akihisa T, Hamasaki Y, Tokuda H, Ukiya M, Kimura Y, Nishino H.

Abstract: Microbial transformation of isosteviol (2), a beyerane-type diterpenoid obtained from stevioside (1) by acid hydrolysis, yielded 7beta-hydroxyisosteviol (3), 11beta-hydroxyisosteviol (5), and 12beta-hydroxyisosteviol (6) by the fungus Aspergillus niger, 17-hydroxyisosteviol (7) by the fungus Glomerella cingulata, and 3 and 7-oxoisosteviol (4) by the fungus Mortierella elongate. The five metabolites, 3-7, along with 1 and 2 were evaluated for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells as a primary screening test for inhibitors of tumor promoters. All the diterpenes tested showed potent inhibitory effects, with the five metabolites 3-7 exhibiting more potent effects.

CiteXplore: 15043419

DOI: 10.1021/np030393q