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ID: ALA1152506
Journal: Bioorg Med Chem
Title: Characterization and synthetic application of a novel beta1,3-galactosyltransferase from Escherichia coli O55:H7.
Authors: Liu XW, Xia C, Li L, Guan WY, Pettit N, Zhang HC, Chen M, Wang PG.
Abstract: A beta1,3-galactosyltransferase (WbgO) was identified in Escherichia coli O55:H7. Its function was confirmed by radioactive activity assay and structure analysis of the disaccharide synthesized with the recombinant enzyme. WbgO requires a divalent metal ion, either Mn(2+) or Mg(2+), for its activity and is active between pH 6.0-8.0 with a pH optimum of 7.0. N-acetylglucosamine (GlcNAc) and oligosaccharides with GlcNAc at the non-reducing end were shown to be its preferred substrates and it can be used for the synthesis of type 1 glycan chains from these substrates. Together with a recombinant bacterial GlcNAc-transferase, benzyl beta-lacto-N-tetraoside was synthesized with the purified WbgO to demonstrate the synthetic utility of WbgO.
CiteXplore: 19560364