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ID: ALA1155051
Journal: Bioorg Med Chem Lett
Title: Simultaneous identification of multiple receptors of natural product using an optimized cDNA phage display cloning.
Authors: He QL, Jiang H, Zhang F, Chen HB, Tang GL.
Abstract: Simultaneously isolating more than one receptor of natural product remains a challenge to chemical genetics. Using cyclosporine A as an affinity probe and an optimized phage display cloning procedure, not only cyclophilin A, but also cyclophilin B was isolated as the full-length gene clone from a human brain cDNA library. This optimized protocol can be used to select protein targets of chemicals dependent on the binding affinity rather than on the relative abundance in cells.
CiteXplore: 18571405