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ID: ALA1156055

Journal: Bioorg Med Chem Lett

Title: Synthetic substrate for application in both high and low throughput assays for botulinum neurotoxin B protease inhibitors.

Authors: Salzameda NT, Barbieri JT, Janda KD.

Abstract: A FRET peptide substrate was synthesized and evaluated for enzymatic cleavage by the BoNT/B light chain protease. The FRET substrate was found to be useful in both a high throughput assay to uncover initial 'hits' and a low throughput HPLC assay to determine kinetic parameters and modes of inhibition.

CiteXplore: 19747823

DOI: 10.1016/j.bmcl.2009.08.079