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ID: ALA1287665
Journal: Bioorg Med Chem
Title: Design, synthesis and inhibitory effect of pentapeptidyl chloromethyl ketones on proteinase K.
Authors: Kore AR, Shanmugasundaram M.
Abstract: The synthesis and proteolytic inhibitor function of new modified pentapeptide MeOSuc-AAAPF-CH(2)Cl 6 is described. The efficacy of 6 in inhibiting the proteolytic activity of proteinase K at a concentration of 0.10 mM allows a signal to be obtained for an exogenous target ('Xeno RNA') at 29 PCR cycles (i.e., Ct=29), whereas the control MeOSuc-AAAPV-CH₂Cl 1 requires a 7.5-fold higher concentration (0.75 mM) to produce the same Ct.
CiteXplore: 20937562