Document Report Card

ID: ALA1649315

Journal: Antimicrob Agents Chemother

Title: Extended-spectrum cephalosporinase in Acinetobacter baumannii.

Authors: Rodríguez-Martínez JM, Nordmann P, Ronco E, Poirel L.

Abstract: An AmpC-type beta-lactamase conferring high-level resistance to expanded-spectrum cephalosporins and monobactams was characterized from an Acinetobacter baumannii clinical isolate. This class C beta-lactamase (named ADC-33) possessed a Pro210Arg substitution together with a duplication of an Ala residue at position 215 (inside the Omega-loop) compared to a reference AmpC cephalosporinase from A. baumannii. ADC-33 hydrolyzed ceftazidime, cefepime, and aztreonam at high levels, which allows the classification of this enzyme as an extended-spectrum AmpC (ESAC). Site-directed mutagenesis confirmed the role of both substitutions in its ESAC property.

CiteXplore: 20547808

DOI: 10.1128/aac.00050-10