Document Report Card

ID: ALA1649546

Journal: Antimicrob Agents Chemother

Title: Extended-spectrum properties of CMY-30, a Val211Gly mutant of CMY-2 cephalosporinase.

Authors: Kotsakis SD, Papagiannitsis CC, Tzelepi E, Tzouvelekis LS, Miriagou V.

Abstract: CMY-30, a Val211Gly mutant of CMY-2 cephalosporinase, was derived by mutagenesis. The hydrolytic efficiency of CMY-30 against expanded-spectrum cephalosporins was higher than that of CMY-2 due to increased k(cat) values. Findings indicate a role of the Omega loop residue 211 in determining the substrate specificities of CMYs also corroborated by modeling studies.

CiteXplore: 19470510

DOI: 10.1128/aac.00219-09