Basic Information
ID: ALA2074110
Journal: J Biochem
Title: Characterization, cDNA cloning, and functional expression of mouse ileal sodium-dependent bile acid transporter.
Authors: Saeki T, Matoba K, Furukawa H, Kirifuji K, Kanamoto R, Iwami K.
Abstract: Mouse ileal sodium dependent bile acid transporter (ISBT) was characterized using isolated enterocytes. Only enterocytes from the most distal portion showed Na+-dependent [3H]taurocholate uptake. Northern blot analysis using a probe against mouse ISBT revealed the expression of mouse ISBT mRNA to be restricted to the distal ileum. The Km and Vmax for Na+-dependent [3H]taurocholate transport into isolated ileocytes were calculated as 27 microM and 360 pmol/mg protein/min, respectively. Uptake of [3H]taurocholate was inhibited by N-ethylmaleimide. We have cloned ISBT cDNA from mouse ileum. The cDNA included the entire open reading frame coding 348 amino acid protein with seven hydrophobic segments and two N-glycosylation sites. COS-7 cells transfected with the expression vector containing this cDNA expressed Na+-dependent [3H]taurocholate uptake activity with a Km of 34 microM.
CiteXplore: 10101301
DOI: 10.1093/oxfordjournals.jbchem.a022358
Patent ID: ┄
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