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ID: ALA2216754
Journal: ACS Med Chem Lett
Title: Discovery of Imidazoquinolines with Toll-Like Receptor 7/8 Independent Cytokine Induction.
Authors: Shi C, Xiong Z, Chittepu P, Aldrich CC, Ohlfest JR, Ferguson DM.
Abstract: Toll-like receptors (TLRs) are key targets in the design of immunomodulating agents for use as vaccine adjuvants and anticancer treatments. The imidazoquinolines, imiquimod and resiquimod, have been shown to activate TLR-7 and -8 which in turn induce cytokine production as part of the innate immune response. Herein, we report the synthesis and discovery of a C7-methoxycarbonyl derivative of imiquimod that stimulates cytokine production but is devoid of TLR-7/8 activity. Data is presented that shows this analog not only induces IL-12p40 and TNF production, similar to that of imiquimod and resiquimod, but greatly enhances the production of IL-1β, a key cytokine involved in activation of CD4 T cells. It is further demonstrated that TLR-7/8 activation can be recovered by the addition of a C2-alkyl substituent to this newly discovered analog. The results support the existence of an alternative mechanism of action by which imidazoquinolines can stimulate cytokine production.
CiteXplore: 22837811
DOI: 10.1021/ml300079e