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ID: ALA4002570

Journal: Eur J Med Chem

Title: Synthesis and cytostatic properties of polyfunctionalized furanoallocolchicinoids.

Authors: Gracheva IA, Voitovich IV, Faerman VI, Sitnikov NS, Myrsikova EV, Schmalz HG, Svirshevskaya EV, Fedorov AY.

Abstract: A series of furan-based allocolchicinoids was prepared from commercially available colchicine via a nine-step reaction sequence. Cytostatic activity, cell cycle arrest, apoptosis, tubulin and F-actin expression were studied in vitro in 2D and 3D cultures of normal and tumor epithelial keratinocytes, endothelial and mesenchymal cells. Among the prepared furanoallocolchicine analogues, 14a and 7a displayed the most pronounced anti-cancer activity. These compounds induced two types of effects: (a) cell cycle arrest in the G2/M phase as a direct consequence of effective tubulin binding (metaphase effect), and (b) pronounced cell stress (as evidenced by the overexpression of tubulin and F-actin), which was caused by the hyperpolarization of mitochondrial and lysosomal membranes (interphase effect).

CiteXplore: 27912174

DOI: 10.1016/j.ejmech.2016.11.020