A new chemotype inhibitor for the human organic cation transporter 3 (hOCT3).

Basic Information

ID: ALA4043181

Journal: Bioorg Med Chem Lett

Title: A new chemotype inhibitor for the human organic cation transporter 3 (hOCT3).

Authors: Pan X, Iyer KA, Liu H, Sweet DH, Dukat M.

Abstract: Human organic cation transporters (OCTs) represent an understudied neurotransmitter uptake mechanism for which no selective agents have yet been identified. Several neurotransmitters (e.g. serotonin, norepinephrine) are low-affinity substrates for these transporters, but possess higher affinity for other transporters (e.g. the serotonin or norepinephrine transporters; SERT and NET, respectively). We have identified a new class of OCT inhibitors with a phenylguanidine structural scaffold. Here, we examine the actions of a series of such compounds and report preliminary structure-activity relationships (SARs) - the first dedicated SAR study of OCT3 action. Initial results showed that the presence of a substituent on the phenyl ring, as well as its position, contributes to the phenylguanidines' inhibitory potency (IC50 values ranging from 2.2 to >450μM) at hOCT3. There is a trend towards enhanced inhibitory potency of phenylguanidines with increased lipophilic character and the size of the substituent at the phenyl 4-position, with the latter reaching a ceiling effect. The first PiPT-based hOCT3 homology models were generated and are in agreement with our biological data.

CiteXplore: 28811134

DOI: 10.1016/j.bmcl.2017.08.008

Patent ID: