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ID: ALA4270485
Journal: J Nat Prod
Title: Determination of Gymnemic Acid I as a Protein Biosynthesis Inhibitor Using Chemical Proteomics.
Authors: Capolupo A, Esposito R, Zampella A, Festa C, Riccio R, Casapullo A, Tosco A, Monti MC.
Abstract: The plant Gymnema sylvestre has been used widely in traditional medicine as a remedy for several diseases, and its leaf extract is known to contain a group of bioactive triterpene saponins belonging to the gymnemic acid class. Gymnemic acid I (1) is one of the main components among this group of secondary metabolites and is endowed with an interesting bioactivity profile. Since there is a lack of information about its specific biological targets, the full interactome of 1 was investigated through a quantitative chemical proteomic approach, based on stable-isotope dimethyl labeling. The ribosome complex was found to be the main partner of compound 1, and a full validation of the proteomics results was achieved by orthogonal approaches. Further biochemical and biological investigations revealed an inhibitory effect of 1 on the ribosome machinery.
CiteXplore: 28256837