Identification of C-6 as a New Site for Linker Conjugation to the Taccalonolide Microtubule Stabilizers.
Basic Information
ID: ALA4422691
Journal: J Nat Prod
Title: Identification of C-6 as a New Site for Linker Conjugation to the Taccalonolide Microtubule Stabilizers.
Authors: Du L, Risinger AL, Yee SS, Ola ARB, Zammiello CL, Cichewicz RH, Mooberry SL.
Abstract: The taccalonolides are a class of microtubule stabilizers that circumvent clinically relevant forms of drug resistance due to their unique mechanism of microtubule stabilization imparted by the covalent binding of the C-22-C-23 epoxide moiety to tubulin. A taccalonolide (8) with a fluorescein group attached with a linker at C-6 was generated, and biochemical and cell-based assays showed that it bound directly to tubulin and stabilized microtubules. This pharmacological probe has allowed, for the first time, a direct visualization of a taccalonolide binding to microtubules, verifying their cellular binding site. This C-6-modified taccalonolide showed potency comparable to the untagged compound in biochemical experiments; however, its potency was lower in cellular assays, presumably due to decreased cellular permeability. These studies provide a valuable tool to facilitate the further understanding of taccalonolide pharmacology and demonstrate that C-6 is a promising site for a linker to be added to this novel class of microtubule stabilizers for targeted drug delivery.
CiteXplore: 30799622
DOI: 10.1021/acs.jnatprod.8b01036
Patent ID: ┄