Document Report Card

ID: ALA4699434

Journal: RSC Med Chem

Title: Fenretinide binding to the lysosomal protein saposin D alters ceramide solubilization and hydrolysis

Authors: Milliken, Brandon T., Melegari, Lindy, Smith, Gideon L., Grohn, Kris, Wolfe, Aaron J., Moody, Kelsey, Bou-Abdallah, Fadi, Doyle, Robert P.

Abstract: Fenretinide is a synthetic retinoid pharmaceutical linked to ceramide build-up in vivo. Saposin D is an intralysosomal protein necessary for ceramide binding/degradation. We show, via electronic absorption spectroscopy, fluorescence spectroscopy, and ceramide hydrolysis assays, that fenretinide is bound by saposin D {Ka = (1.45 +/- 0.49) x 105 M-1}, and affects ceramide solubilization/degradation.

CiteXplore: 33479697

DOI: 10.1039/d0md00182a