Document Report Card

Basic Information

ID: ALA4706482

Journal: Eur J Med Chem

Title: Structural optimization of aminopyrimidine-based CXCR4 antagonists.

Authors: Zhu,F.; Wang,Y.; Du,Q.; Ge,W.; Li,Z.; Wang,X.; Fu,C.; Luo,L.; Tian,S.; Ma,H.; Zheng,J.; Zhang,Y.; Sun,X.; He,S.; Zhang,X.

Abstract: Structural optimization of aminopyrimidine-based CXCR4 antagonists is reported. The optimization is guided by molecular docking studies based on available CXCR4-small molecule crystal complex. The optimization identifies a number of compounds with improved receptor binding affinity and functional activity exemplified by compound 23 (inhibition of APC-conjugate clone 12G5 for CXCR4 binding in a cell based assay: IC = 8.8 nM; inhibition of CXCL12 induced cytosolic calcium increase: IC = 0.02 nM). In addition, compound 23 potently inhibits CXCR4/CXLC12 mediated chemotaxis in a matrigel invasion assay. Furthermore, compound 23 exhibits good physicochemical properties (MW 367, clogP 2.1, PSA 48, pKa 7.2) and in vitro safety profiles (marginal/moderate inhibition of CYP isozymes and hERG). These results represent significant improvement over the initial hit from scaffold hybridization and suggest that compound 23 can be used as a starting point to support lead optimization.

CiteXplore: 31806538

DOI: 10.1016/j.ejmech.2019.111914